Application | Comment | Organism |
---|---|---|
synthesis | recombinant enzyme produced in Escherichia coli can efficiently convert colloidal chitin to N-acetyl glucosamine and chitobiose at pH 4.0, 6.0 and 9.0 at 50°C and retains its activity up to 3 days under these conditions | Bacillus licheniformis |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Bacillus licheniformis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
4-nitrophenyl beta-N,N'-diacetylchitobioside | pH 6.0, 37°C | Bacillus licheniformis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Bacillus licheniformis | 5829 | - |
periplasm | - |
Bacillus licheniformis | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
66000 | - |
x * 66801, calculated, x * 66000, SDS-PAGE | Bacillus licheniformis |
66801 | - |
x * 66801, calculated, x * 66000, SDS-PAGE | Bacillus licheniformis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus licheniformis | B7UB89 | isoform ChiA; strain DSM8785 | - |
Bacillus licheniformis DSM 8785 | B7UB89 | isoform ChiA; strain DSM8785 | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.294 | - |
substrate 4-nitrophenyl-chitobiose, pH 6.0, 37°C | Bacillus licheniformis |
Storage Stability | Organism |
---|---|
-25°C, 20% glycerol, up to one year with more than 80% residual activity | Bacillus licheniformis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl beta-N,N'-diacetylchitobioside + H2O | - |
Bacillus licheniformis | 4-nitrophenol + N,N'-diacetylchitobiose | - |
? | |
4-nitrophenyl beta-N,N'-diacetylchitobioside + H2O | - |
Bacillus licheniformis DSM 8785 | 4-nitrophenol + N,N'-diacetylchitobiose | - |
? | |
colloidal chitin + H2O | - |
Bacillus licheniformis | N-acetyl-D-glucosamine + N,N'-diacetylchitobiose | - |
? | |
colloidal chitin + H2O | - |
Bacillus licheniformis DSM 8785 | N-acetyl-D-glucosamine + N,N'-diacetylchitobiose | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 66801, calculated, x * 66000, SDS-PAGE | Bacillus licheniformis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.31 | - |
4-nitrophenyl beta-N,N'-diacetylchitobioside | pH 6.0, 37°C | Bacillus licheniformis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Bacillus licheniformis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | 11 | - |
Bacillus licheniformis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
10.3 | - |
4-nitrophenyl-chitobiose | pH 6.0, 37°C | Bacillus licheniformis |