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Literature summary for 3.2.1.14 extracted from

  • Lopez, R.C.; Gomez-Gomez, L.
    Isolation of a new fungi and wound-induced chitinase class in corms of Crocus sativus (2009), Plant Physiol. Biochem., 47, 426-434.
    View publication on PubMed
Search for more literature for 3.2.1.14

Activating Compound

Activating Compound Comment Organism Structure
additional information the chitinase encoded by gene chiA is induced by elicitor treatment, methyl jasmonate, wounding, and by the fungi Fusarium oxysporum, Beauveria and Phoma sp. Crocus sativus

Cloned(Commentary)

Cloned (Comment) Organism
gene SafchiA, DNA and amino acid sequence determination and analysis, phylogenetic tree, RT-PCR expression analysis, expression of GST-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3), the recombinant wild-type enzyme shows chitinolytic and antifungal activity Crocus sativus

Protein Variants

Protein Variants Comment Organism
E145L site-directed mutagenesis, the mutant retains approximately 86% activity of wild-type activity Crocus sativus
E145L/E155A site-directed mutagenesis of a catalytic residue, inactive mutant Crocus sativus
E155A site-directed mutagenesis of a catalytic residue, inactive mutant Crocus sativus
T133E site-directed mutagenesis, the mutant shows slightly increased catalytic activity compared to the wild-type enzyme Crocus sativus

Organism

Organism UniProt Comment Textmining
Crocus sativus B5L6N2 gene SafchiA
-

Purification (Commentary)

Purification (Comment) Organism
recombinant GST-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by glutathione affinity chromatography Crocus sativus

Source Tissue

Source Tissue Comment Organism Textmining
corm
-
 Crocus sativus
-
root
-
 Crocus sativus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CM-chitin-RBV + H2O polymeric substrate Crocus sativus ?
-
 ?
additional information residue Glu155 is involved in the catalytic reaction Crocus sativus ?
-
 ?

Synonyms

Synonyms Comment Organism
More ChiA belongs to family 19 of glycosyl hydrolases Crocus sativus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 chitinolytic assay at Crocus sativus
45
-
 antifungal activity assay at Crocus sativus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
 assay at Crocus sativus