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Literature summary for 3.2.1.14 extracted from
- Characterization of chitinases of polycentric anaerobic rumen fungi (2008), Folia Microbiol. (Praha), 53, 241-245.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| iodoacetamide | 10% activation at 1 mM, no activation at 10 mM | Orpinomyces sp. |  |
| iodoacetamide | 19% activation at 1 mM, 12% inhibition at 10 mM | Anaeromyces mucronatus | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | slight inhibition at 10 mM | Anaeromyces mucronatus | |
| 1,10-phenanthroline | slight inhibition at 10 mM | Orpinomyces sp. | |
| 2-sufanylethanol | - |
Anaeromyces mucronatus | |
| 2-sufanylethanol | - |
Orpinomyces sp. | |
| Co2+ | - |
Anaeromyces mucronatus | |
| Co2+ | - |
Orpinomyces sp. | |
| DTT | - |
Anaeromyces mucronatus | |
| DTT | - |
Orpinomyces sp. | |
| EDTA | - |
Anaeromyces mucronatus | |
| EDTA | - |
Orpinomyces sp. | |
| Hg2+ | - |
Anaeromyces mucronatus | |
| Hg2+ | - |
Orpinomyces sp. | |
| iodoacetamide | 19% activation at 1 mM, 12% inhibition at 10 mM | Anaeromyces mucronatus | |
| iodoacetic acid | slight inhibition at 10 mM | Anaeromyces mucronatus | |
| iodoacetic acid | slight inhibition at 10 mM | Orpinomyces sp. | |
| K+ | slightly activating at 1 mM, slightly inhibitory at 10 mM | Anaeromyces mucronatus | |
| L-cysteine | - |
Anaeromyces mucronatus | |
| L-cysteine | - |
Orpinomyces sp. | |
| Mg2+ | slightly activating at 1 mM, slightly inhibitory at 10 mM | Anaeromyces mucronatus | |
| Mn2+ | - |
Anaeromyces mucronatus | |
| Mn2+ | - |
Orpinomyces sp. | |
| PMSF | - |
Anaeromyces mucronatus | |
| PMSF | - |
Orpinomyces sp. | |
| SDS | inhibits 95% of activity at 10 mM | Anaeromyces mucronatus | |
| SDS | inhibits completely at 10 mM | Orpinomyces sp. | |
| thimerosal | - |
Anaeromyces mucronatus | |
| thimerosal | - |
Orpinomyces sp. | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Orpinomyces sp. | 5829 | - |
| cytosol | - |
Anaeromyces mucronatus | 5829 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ba2+ | activates | Orpinomyces sp. | |
| Ba2+ | activates | Anaeromyces mucronatus | |
| Fe3+ | activates | Orpinomyces sp. | |
| Fe3+ | activates | Anaeromyces mucronatus | |
| K+ | slightly activating at 1 mM, slightly inhibitory at 10 mM | Anaeromyces mucronatus | |
| Mg2+ | activates | Orpinomyces sp. | |
| Mg2+ | slightly activating at 1 mM, slightly inhibitory at 10 mM | Anaeromyces mucronatus | |
| additional information | the enzyme activity is not affected by K+ at up to 10 mM | Orpinomyces sp. |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| chitin + H2O | Orpinomyces sp. | - |
? | - |
? | |
| chitin + H2O | Anaeromyces mucronatus | - |
? | - |
? | |
| chitin + H2O | Orpinomyces sp. KF7 | - |
? | - |
? | |
| chitin + H2O | Anaeromyces mucronatus KF8 | - |
? | - |
? | |
| chitosan + H2O | Orpinomyces sp. | - |
? | - |
? | |
| chitosan + H2O | Anaeromyces mucronatus | - |
? | - |
? | |
| chitosan + H2O | Orpinomyces sp. KF7 | - |
? | - |
? | |
| chitosan + H2O | Anaeromyces mucronatus KF8 | - |
? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Anaeromyces mucronatus | - |
isolated from rumen fluid of fistulated cow | - |
| Anaeromyces mucronatus KF8 | - |
isolated from rumen fluid of fistulated cow | - |
| Orpinomyces sp. | - |
isolated from rumen fluid of fistulated cow | - |
| Orpinomyces sp. KF7 | - |
isolated from rumen fluid of fistulated cow | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| mycelium | - |
Orpinomyces sp. | - |
| mycelium | - |
Anaeromyces mucronatus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl di-N,N'-acetyl-beta-D-chitobiose + H2O | - |
Orpinomyces sp. | ? | - |
? | |
| 4-nitrophenyl di-N,N'-acetyl-beta-D-chitobiose + H2O | - |
Anaeromyces mucronatus | ? | - |
? | |
| 4-nitrophenyl di-N,N'-acetyl-beta-D-chitobiose + H2O | - |
Orpinomyces sp. KF7 | ? | - |
? | |
| 4-nitrophenyl di-N,N'-acetyl-beta-D-chitobiose + H2O | - |
Anaeromyces mucronatus KF8 | ? | - |
? | |
| chitin + H2O | - |
Orpinomyces sp. | ? | - |
? | |
| chitin + H2O | - |
Anaeromyces mucronatus | ? | - |
? | |
| chitin + H2O | fungal cell-wall chitin is the preferred substrate, the enzyme is also active with CM-, DDD-, colloidal chitin, and chitin from cattle fish, substrate specificity, overview | Orpinomyces sp. | ? | - |
? | |
| chitin + H2O | fungal cell-wall chitin is the preferred substrate, the enzyme is also active with CM-, DDD-, colloidal chitin, and chitin from cattle fish, substrate specificity, overview | Anaeromyces mucronatus | ? | - |
? | |
| chitin + H2O | - |
Orpinomyces sp. KF7 | ? | - |
? | |
| chitin + H2O | fungal cell-wall chitin is the preferred substrate, the enzyme is also active with CM-, DDD-, colloidal chitin, and chitin from cattle fish, substrate specificity, overview | Orpinomyces sp. KF7 | ? | - |
? | |
| chitin + H2O | - |
Anaeromyces mucronatus KF8 | ? | - |
? | |
| chitin + H2O | fungal cell-wall chitin is the preferred substrate, the enzyme is also active with CM-, DDD-, colloidal chitin, and chitin from cattle fish, substrate specificity, overview | Anaeromyces mucronatus KF8 | ? | - |
? | |
| chitosan + H2O | - |
Orpinomyces sp. | ? | - |
? | |
| chitosan + H2O | - |
Anaeromyces mucronatus | ? | - |
? | |
| chitosan + H2O | chitosan-succinate is the preferred substrate, the enzyme is also active with CM-chitosan, colloidal chitosan, chtiosan-asparatae and chitosan-glutamate, substrate specificity, overview | Orpinomyces sp. | ? | - |
? | |
| chitosan + H2O | chitosan-succinate is the preferred substrate, the enzyme is also active with CM-chitosan, colloidal chitosan, chtiosan-asparatae and chitosan-glutamate, substrate specificity, overview | Anaeromyces mucronatus | ? | - |
? | |
| chitosan + H2O | - |
Orpinomyces sp. KF7 | ? | - |
? | |
| chitosan + H2O | chitosan-succinate is the preferred substrate, the enzyme is also active with CM-chitosan, colloidal chitosan, chtiosan-asparatae and chitosan-glutamate, substrate specificity, overview | Orpinomyces sp. KF7 | ? | - |
? | |
| chitosan + H2O | - |
Anaeromyces mucronatus KF8 | ? | - |
? | |
| chitosan + H2O | chitosan-succinate is the preferred substrate, the enzyme is also active with CM-chitosan, colloidal chitosan, chtiosan-asparatae and chitosan-glutamate, substrate specificity, overview | Anaeromyces mucronatus KF8 | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| endochitinase | - |
Orpinomyces sp. |
| endochitinase | - |
Anaeromyces mucronatus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Anaeromyces mucronatus |
| 50 | - |
- |
Orpinomyces sp. |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
stable up to | Anaeromyces mucronatus |
| 50 | - |
stable up to | Orpinomyces sp. |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
- |
Orpinomyces sp. |
| 6.5 | - |
- |
Anaeromyces mucronatus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 2.5 | 8 | - |
Orpinomyces sp. |
| 2.5 | 8 | - |
Anaeromyces mucronatus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 7 | - |
Orpinomyces sp. |
| 4.5 | 7 | - |
Anaeromyces mucronatus |
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