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Literature summary for 3.2.1.14 extracted from
- Degradation of chitosans with chitinase G from Streptomyces coelicolor A3(2): production of chito-oligosaccharides and insight into subsite specificities (2009), Biomacromolecules, 10, 892-899.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of ChiG in Escherichia coli | Streptomyces coelicolor |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces coelicolor | - |
11 putative family 18 chitinases and two putative family 19 chitinases, ChiF and ChiG | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant ChiG from Escherichia coli | Streptomyces coelicolor |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins | mode of action of ChiG, family 19 chitinases use a single-displacement reaction with inversion of the configuration of the anomeric carbon, overview | Streptomyces coelicolor |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
substrate specificity, overview | Streptomyces coelicolor |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| chitooligosaccharides + H2O | degradation of a 64% acetylated chitosan yields a continuum of oligomers, showing that ChiG operates according to a nonprocessive, endo mode of action | Streptomyces coelicolor | ? | - |
? |  |
| chitosan + H2O | - |
Streptomyces coelicolor | ? | - |
? | |
| additional information | substrate specificity, overview | Streptomyces coelicolor | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ChiG | - |
Streptomyces coelicolor |
| chitinase G | - |
Streptomyces coelicolor |
| additional information | ChiG belongs to the bacterial family 19 of glycohydrolases | Streptomyces coelicolor |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Streptomyces coelicolor |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
assay at | Streptomyces coelicolor |
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