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Literature summary for 3.2.1.14 extracted from

  • Fukamizo, T.; Miyake, R.; Tamura, A.; Ohnuma, T.; Skriver, K.; Pursiainen, N.V.; Juffer, A.H.
    A flexible loop controlling the enzymatic activity and specificity in a glycosyl hydrolase family 19 endochitinase from barley seeds (Hordeum vulgare L.) (2009), Biochim. Biophys. Acta, 1794, 1159-1167.
    View publication on PubMed
Search for more literature for 3.2.1.14

Protein Variants

Protein Variants Comment Organism
W72A the mutation affects the thermal stability and specific activities toward glycol chitin and chitin hexasaccharide compared to the wild-type enzyme Hordeum vulgare
W72A/W82A the double mutation affects the thermal stability and specific activities toward glycol chitin and chitin hexasaccharide compared to the wild-type enzyme, but the effect lies between the affection of the individual mutations Hordeum vulgare
W82A the mutation affects the thermal stability and specific activities toward glycol chitin and chitin hexasaccharide compared to the wild-type enzyme Hordeum vulgare

Organism

Organism UniProt Comment Textmining
Hordeum vulgare P23951
-
-

Source Tissue

Source Tissue Comment Organism Textmining
seed
-
 Hordeum vulgare
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 no activity of mutant W72A/W82A with substrate GlcNAc hexasaccharide Hordeum vulgare
0.02
-
 mutant W82A, substrate glycol chitin Hordeum vulgare
0.38
-
 mutant W72A/W82A, substrate glycol chitin Hordeum vulgare
2.53
-
 mutant W72A, substrate glycol chitin Hordeum vulgare
6.31
-
 wild-type enzyme, substrate glycol chitin Hordeum vulgare
23.4
-
 mutant W82A, substrate GlcNAc hexasaccharide Hordeum vulgare
139
-
 wild-type enzyme, substrate GlcNAc hexasaccharide Hordeum vulgare
251
-
 mutant W72A, substrate GlcNAc hexasaccharide Hordeum vulgare

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chitin + H2O a flexible loop controlling the enzymatic activity and specificity in the glycosyl hydrolase family 19 endochitinase from barley seeds Hordeum vulgare ?
-
 ?
GlcNAcbeta(1-4)GlcNAc + H2O
-
 Hordeum vulgare ?
-
 ?
GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
-
 Hordeum vulgare ?
-
 ?
GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
-
 Hordeum vulgare ?
-
 ?
GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
-
 Hordeum vulgare ?
-
 ?
GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
-
 Hordeum vulgare ?
-
 ?

Subunits

Subunits Comment Organism
More molecular dynamics simulations of wild-type and mutant enzymes, overview Hordeum vulgare

Synonyms

Synonyms Comment Organism
endochitinase
-
 Hordeum vulgare
More the endochitinase belongs to the glycosyl hydrolase family 19 Hordeum vulgare

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25 40 assay at Hordeum vulgare