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Literature summary for 3.2.1.14 extracted from
- Purification and characterization of thermostable chitinase from Bacillus licheniformis SK-1 (2009), Appl. Biochem. Biotechnol., 157, 23-35.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Chi72 follows the classical Michaelis-Menten kinetics | Bacillus licheniformis | |
| 0.23 | - |
colloidal chitin | - |
Bacillus licheniformis |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 72000 | - |
x * 72000, Chi72, SDS-PAGE | Bacillus licheniformis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus licheniformis | - |
isolated from soil in Angthong Province, Thailand | - |
| Bacillus licheniformis SK-1 | - |
isolated from soil in Angthong Province, Thailand | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme 11.5fold from strain SK-1 by colloidal chitin affinity and anion exchange chromatography | Bacillus licheniformis |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2 | 2.5 | purified enzyme | Bacillus licheniformis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| chitin + H2O | colloidal , regenerated, or partially N-acetylated chitin substrates | Bacillus licheniformis | ? | - |
? |  |
| chitin + H2O | colloidal , regenerated, or partially N-acetylated chitin substrates | Bacillus licheniformis SK-1 | ? | - |
? | |
| chitosan + H2O | - |
Bacillus licheniformis | ? | - |
? | |
| chitosan + H2O | - |
Bacillus licheniformis SK-1 | ? | - |
? | |
| colloidal chitin + H2O | - |
Bacillus licheniformis | ? | - |
? | |
| colloidal chitin + H2O | - |
Bacillus licheniformis SK-1 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 72000, Chi72, SDS-PAGE | Bacillus licheniformis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Chi72 | - |
Bacillus licheniformis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
Chi72 | Bacillus licheniformis |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 70 | active range, Chi72 | Bacillus licheniformis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
4 h, purified Chi72, stable | Bacillus licheniformis |
| 50 | - |
2 h, purified Chi72, stable | Bacillus licheniformis |
| 60 | - |
half-life 90 min, purified Chi72 | Bacillus licheniformis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
maximum 1 of Chi72 | Bacillus licheniformis |
| 8 | - |
maximum 2 of Chi72 | Bacillus licheniformis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 9 | - |
Bacillus licheniformis |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 9 | 24 h, purified Chi72, stable | Bacillus licheniformis |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Bacillus licheniformis | Chi72 | - |
4.62 |
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