Cloned (Comment) | Organism |
---|---|
C-terminal truncated enzyme, ChiCWDELTAFC, is expressed in Escherichia coli | Bacillus cereus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | C-terminal truncated enzyme, ChiCWDELTAFC, is expressed in Escherichia coli and purified to homogeneity for biochemical characterization. Compared with ChiCW, ChiCWDELTAFC exhibits higher chitinase activity at high temperature and pH, but expresses lower hydrolytic and binding activities toward insoluble substrates. ChiCWDELTAFC hydrolyzes oligomeric and polymeric substrates less efficiently than ChiCW | Bacillus cereus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | KM-value for glycol chitin at 37°C is 1.9 mg/ml, C-terminal truncated enzyme ChiCWDELTAFC. KM-value for colloidal chitin at 37°C is 13.7 mg/ml, C-terminal truncated enzyme ChiCWDELTAFC | Bacillus cereus | |
0.0553 | - |
4-methyl-2-oxo-2H-chromen-7-yl 2-(acetylamino)-4-O-[2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl]-2-deoxy-beta-D-glucopyranoside | 37°C, C-terminal truncated enzyme ChiCWDELTAFC | Bacillus cereus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
the C-terminal truncated enzyme ChiCWDELTAFC has a calculated molecular weight of 50573 Da | Bacillus cereus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus cereus | - |
- |
- |
Bacillus cereus 28-9 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
C-terminal truncated enzyme, ChiCWDELTAFC, expressed in Escherichia coli | Bacillus cereus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-methyl-2-oxo-2H-chromen-7-yl 2-(acetylamino)-4-O-[2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl]-2-deoxy-beta-D-glucopyranoside + H2O | - |
Bacillus cereus | ? | - |
? | |
4-methylumbelliferyl N,N',N''-triacetylchitotriose + H2O | - |
Bacillus cereus | 4-methylumbelliferone + N,N',N''-triacetylchitotriose | - |
? | |
4-methylumbelliferyl N,N',N''-triacetylchitotriose + H2O | - |
Bacillus cereus 28-9 | 4-methylumbelliferone + N,N',N''-triacetylchitotriose | - |
? | |
chitin + H2O | - |
Bacillus cereus | ? | - |
? | |
chitin + H2O | - |
Bacillus cereus 28-9 | ? | - |
? | |
chitosan + H2O | - |
Bacillus cereus | ? | - |
? | |
chitosan + H2O | - |
Bacillus cereus 28-9 | ? | - |
? | |
colloidal chitin + H2O | - |
Bacillus cereus | ? | - |
? | |
colloidal chitin + H2O | - |
Bacillus cereus 28-9 | ? | - |
? | |
glycol chitin + H2O | - |
Bacillus cereus | ? | - |
? | |
glycol chitin + H2O | - |
Bacillus cereus 28-9 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ChiCW | - |
Bacillus cereus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.075 | - |
glycol chitin | 37°C, C-terminal truncated enzyme ChiCWDELTAFC | Bacillus cereus | |
0.9 | - |
4-methyl-2-oxo-2H-chromen-7-yl 2-(acetylamino)-4-O-[2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl]-2-deoxy-beta-D-glucopyranoside | 37°C, C-terminal truncated enzyme ChiCWDELTAFC | Bacillus cereus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Bacillus cereus | the C-terminal truncated enzyme ChiCWDELTAFC has a calculated pI-value of 5.75 | - |
additional information |