Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Bacillus sp. (in: Bacteria) |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | 5 mM, 95% inhibition | Bacillus sp. (in: Bacteria) | |
EDTA | 10 mM, 15% inhibition | Bacillus sp. (in: Bacteria) | |
Hg2+ | 1 mM, complete inhibition | Bacillus sp. (in: Bacteria) | |
Zn2+ | 5 mM, complete inhibition | Bacillus sp. (in: Bacteria) |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | 5 mM, 1.36fold increase in activity | Bacillus sp. (in: Bacteria) | |
Ca2+ | 5 mM, 1.25fold increase in activity | Bacillus sp. (in: Bacteria) | |
Co2+ | 5 mM, 1.43fold increase in activity | Bacillus sp. (in: Bacteria) | |
Li+ | 5 mM, 1.2fold increase in activity | Bacillus sp. (in: Bacteria) | |
Mg2+ | 5 mM, 1.24fold increase in activity | Bacillus sp. (in: Bacteria) |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus sp. (in: Bacteria) | A0EQW8 | - |
- |
Bacillus sp. (in: Bacteria) DAU101 | A0EQW8 | - |
- |
Purification (Comment) | Organism |
---|---|
GST-fusion purification system | Bacillus sp. (in: Bacteria) |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
chitin + H2O | - |
Bacillus sp. (in: Bacteria) | N,N'-diacetylchitobiose + ? | - |
? | |
chitin + H2O | - |
Bacillus sp. (in: Bacteria) DAU101 | N,N'-diacetylchitobiose + ? | - |
? | |
chitosan + H2O | - |
Bacillus sp. (in: Bacteria) | ? | - |
? | |
chitosan + H2O | - |
Bacillus sp. (in: Bacteria) DAU101 | ? | - |
? | |
additional information | the enzyme can not hydrolyze glycol chitin or glycol chitosan | Bacillus sp. (in: Bacteria) | ? | - |
? | |
additional information | the enzyme can not hydrolyze glycol chitin or glycol chitosan | Bacillus sp. (in: Bacteria) DAU101 | ? | - |
? | |
N,N'-diacetylchitobiose + H2O | - |
Bacillus sp. (in: Bacteria) | N-acetylglucosamine | - |
? | |
N,N'-diacetylchitobiose + H2O | - |
Bacillus sp. (in: Bacteria) DAU101 | N-acetylglucosamine | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Bacillus sp. (in: Bacteria) |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Bacillus sp. (in: Bacteria) |