Cloned (Comment) | Organism |
---|---|
- |
Vibrio proteolyticus |
Crystallization (Comment) | Organism |
---|---|
expression in Escherichia coli | Vibrio proteolyticus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
1 * 60000, SDS-PAGE | Vibrio proteolyticus |
60088 | - |
1 * 60088, calculated from sequence | Vibrio proteolyticus |
74000 | - |
gel filtration | Vibrio proteolyticus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Vibrio proteolyticus | Q25BT4 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Vibrio proteolyticus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
363.2 | - |
substrate: N,N',N'',N''',N'''',N'''''-hexaacetylchitohexaose | Vibrio proteolyticus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-chitin + H2O | weak activity | Vibrio proteolyticus | ? | - |
? | |
chitin + H2O | weak activity | Vibrio proteolyticus | ? | - |
? | |
ethylene glycol chitin + H2O | weak activity | Vibrio proteolyticus | ? | - |
? | |
additional information | no hydrolysis of p-nitrophenyl-N,N'-diacetylchitobiose, N,N'-diacetylchitobiose and N,N',N''-triacetylchitotriose. Activity with N,N',N'',N''',N''''-pentaacetylchitopentaose is 0.1% of the activity with N,N',N'',N''',N'''',N'''''-hexaacetylchitohexaose | Vibrio proteolyticus | ? | - |
? | |
N,N',N'',N''',N'''',N'''''-hexaacetylchitohexaose + H2O | - |
Vibrio proteolyticus | N,N'-diacetylchitobiose + N,N',N'',N''',N''''-pentaacetylchitopentaose | - |
? | |
N,N',N'',N''',N''''-pentaacetylchitopentaose + H2O | hydrolyzed at 27.8% of the activity with N,N',N'',N''',N'''',N'''''-hexaacetylchitohexaose. The initial alpha-anomer ratio of N,N',N'',N''',N''''-pentaacetylchitopentaose is 59%. After the hydrolysis of N,N',N'',N''',N''''-pentaacetylchitopentaose, only the alpha-anomer of N,N',N''-triacetylchitotriose is found after the reaction for 1 min | Vibrio proteolyticus | N,N'-diacetylchitobiose + N,N',N''-triacetylchitotriose | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 60000, SDS-PAGE | Vibrio proteolyticus |
monomer | 1 * 60088, calculated from sequence | Vibrio proteolyticus |
Synonyms | Comment | Organism |
---|---|---|
Chi19 | - |
Vibrio proteolyticus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Vibrio proteolyticus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | 7 | - |
Vibrio proteolyticus |