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Literature summary for 3.2.1.14 extracted from

  • Park, S.K.; Kim, C.W.; Kim, H.; Jung, J.S.; Harman, G.E.
    Cloning and high-level production of a chitinase from Chromobacterium sp. and the role of conserved or nonconserved residues on its catalytic activity (2007), Appl. Microbiol. Biotechnol., 74, 791-804.
    View publication on PubMed
Search for more literature for 3.2.1.14

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type enzyme and mutant enzymes in Escherichia coli Chromobacterium sp.

Protein Variants

Protein Variants Comment Organism
D271E strain expressing the mutant enzyme is unable to clear colloidal chitin Chromobacterium sp.
D271W mutant enzyme shows 0.09% of the chitinolytic activity as compared to wild-type enzyme Chromobacterium sp.
E273W mutant enzyme shows 0.01% of the chitinolytic activity as compared to wild-type enzyme Chromobacterium sp.
E273W strain expressing the mutant enzyme is unable to clear colloidal chitin Chromobacterium sp.
G215E mutant enzyme shows 0.05% of the chitinolytic activity as compared to wild-type enzyme Chromobacterium sp.
G215E strain expressing the mutant enzyme is unable to clear colloidal chitin Chromobacterium sp.
G216E mutant enzyme shows 0.04% of the chitinolytic activity as compared to wild-type enzyme Chromobacterium sp.
G216E strain expressing the mutant enzyme is unable to clear colloidal chitin Chromobacterium sp.
G403E mutant enzyme shows 0.16% of the chitinolytic activity as compared to wild-type enzyme Chromobacterium sp.
G403E strain expressing the mutant enzyme is unable to clear colloidal chitin Chromobacterium sp.
G492E mutant enzyme shows 0.18% of the chitinolytic activity as compared to wild-type enzyme Chromobacterium sp.
G492E strain expressing the mutant enzyme is unable to clear colloidal chitin Chromobacterium sp.
I270L mutant enzyme shows 80% of the chitinolytic activity as compared to wild-type enzyme Chromobacterium sp.
I270V mutant enzyme shows 58% of the chitinolytic activity as compared to wild-type enzyme Chromobacterium sp.
N401V mutant enzyme shows 97% of the chitinolytic activity as compared to wild-type enzyme Chromobacterium sp.
S213E mutant enzyme shows 0.13% of the chitinolytic activity as compared to wild-type enzyme Chromobacterium sp.
S213E strain expressing the mutant enzyme is unable to clear colloidal chitin Chromobacterium sp.
S497E mutant enzyme shows 30% of the chitinolytic activity as compared to wild-type enzyme Chromobacterium sp.
T218S mutant enzyme shows 173% of the chitinolytic activity as compared to wild-type enzyme Chromobacterium sp.
T218S mutant has about 133% of the activity of the wild type, based on comparison of Kcat values Chromobacterium sp.
V493M mutant enzyme shows 48% of the chitinolytic activity as compared to wild-type enzyme Chromobacterium sp.
W496E mutant enzyme shows 0.75% of the chitinolytic activity as compared to wild-type enzyme Chromobacterium sp.
W496E strain expressing the mutant enzyme is unable to clear colloidal chitin Chromobacterium sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.034
-
 4-methylumbelliferyl-N,N'-diacetylchitobiose pH 7.0, 37°C, mutant enzyme T218S Chromobacterium sp.
0.035
-
 4-methylumbelliferyl-(GlcNAc) pH 7.0, 37°C, mutant enzyme I270V Chromobacterium sp.
0.035
-
 4-methylumbelliferyl-N,N'-diacetylchitobiose pH 7.0, 37°C, mutant enzyme S497E Chromobacterium sp.
0.036
-
 4-methylumbelliferyl-N,N'-diacetylchitobiose pH 7.0, 37°C, wild-type enzyme Chromobacterium sp.
0.036
-
 4-methylumbelliferyl-N,N'-diacetylchitobiose pH 7.0, 37°C, mutant enzyme I270L Chromobacterium sp.
0.036
-
 4-methylumbelliferyl-N,N'-diacetylchitobiose pH 7.0, 37°C, mutant enzyme V493M Chromobacterium sp.
0.042
-
 4-methylumbelliferyl-N,N'-diacetylchitobiose pH 7.0, 37°C, mutant enzyme N401V Chromobacterium sp.

Organism

Organism UniProt Comment Textmining
Chromobacterium sp. Q5MYT4
-
-
Chromobacterium sp. C-61 Q5MYT4
-
-

Source Tissue

Source Tissue Comment Organism Textmining
culture supernatant
-
 Chromobacterium sp.
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylumbelliferyl-(GlcNAc) + H2O
-
 Chromobacterium sp. 4-methylumbelliferone + N-acetyl-D-glucosamine
-
 ?
4-methylumbelliferyl-(GlcNAc) + H2O
-
 Chromobacterium sp. C-61 4-methylumbelliferone + N-acetyl-D-glucosamine
-
 ?
4-methylumbelliferyl-N,N'-diacetylchitobiose + H2O
-
 Chromobacterium sp. ?
-
 ?
4-methylumbelliferyl-N,N'-diacetylchitobiose + H2O
-
 Chromobacterium sp. C-61 ?
-
 ?
chitin + H2O colloidal chitin and crystal chitin Chromobacterium sp. ?
-
 ?
chitin + H2O colloidal chitin and crystal chitin Chromobacterium sp. C-61 ?
-
 ?

Synonyms

Synonyms Comment Organism
Chi54
-
 Chromobacterium sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
7
-
 4-methylumbelliferyl-N,N'-diacetylchitobiose pH 7.0, 37°C, mutant enzyme S497E Chromobacterium sp.
9
-
 4-methylumbelliferyl-N,N'-diacetylchitobiose pH 7.0, 37°C, mutant enzyme I270V Chromobacterium sp.
9
-
 4-methylumbelliferyl-N,N'-diacetylchitobiose pH 7.0, 37°C, mutant enzyme V493M Chromobacterium sp.
13
-
 4-methylumbelliferyl-N,N'-diacetylchitobiose pH 7.0, 37°C, mutant enzyme I270L Chromobacterium sp.
18
-
 4-methylumbelliferyl-N,N'-diacetylchitobiose pH 7.0, 37°C, wild-type enzyme Chromobacterium sp.
20
-
 4-methylumbelliferyl-N,N'-diacetylchitobiose pH 7.0, 37°C, mutant enzyme N401V Chromobacterium sp.
24
-
 4-methylumbelliferyl-N,N'-diacetylchitobiose pH 7.0, 37°C, mutant enzyme T218S Chromobacterium sp.