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Literature summary for 3.2.1.14 extracted from

  • Chou, Y.T.; Yao, S.; Czerwinski, R.; Fleming, M.; Krykbaev, R.; Xuan, D.; Zhou, H.; Brooks, J.; Fitz, L.; Strand, J.; Presman, E.; Lin, L.; Aulabaugh, A.; Huang, X.
    Kinetic characterization of recombinant human acidic mammalian chitinase (2006), Biochemistry, 45, 4444-4454.
    View publication on PubMed
Search for more literature for 3.2.1.14

Cloned(Commentary)

Cloned (Comment) Organism
gene CHIA, expression of His-tagged AMCase in CHO cells Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information detailed kinetics of recombinant AMCase, steady-state kinetics,kinetic mechanism Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
additional information ionic strength modulates substrate cleavage pattern and activity of AMCase with fluorogenic substrates, overview Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
chitin + H2O Homo sapiens
-
 ?
-
 r
additional information Homo sapiens AMCase is important in cytokine Th2-mediated inflammation and is implicated in asthma and allergic disease, inhibition of AMCase results in increased airway inflammation and airway hyperresponsiveness, the enzyme's transglycosylation activity probably is a mechanism in vivo for feedback regulation of the chitinase activity ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q9BZP6 precursor; gene CHIA
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged AMCase from CHO cells by nickel affinity and hydrophobic interaction chromatography, and gel filtration Homo sapiens

Reaction

Reaction Comment Organism Reaction ID
chitopentaose + H2O = chitobiose + chitotriose catalytic reaction mechanism involving Glu119, overview Homo sapiens
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(GlcNAc)6 + H2O the enzyme prefers the beta-anomer of chito-oligosaccharides as substrate, cleavage of beta-glycosidic linkages from the non-reducing end primarily by disaccharide units Homo sapiens (GlcNAc)2 + (GlcNAc)3 + (GlcNAc)4 product analysis r
4-methylumbelliferyl beta-N,N',N''-triacetylchitotrioside + H2O
-
 Homo sapiens 4-methylumbelliferol + beta-N,N',N''-triacetylchitotriose product analysis ?
4-methylumbelliferyl beta-N,N'-diacetylchitobioside + H2O
-
 Homo sapiens 4-methylumbelliferol + beta-N,N'-diacetylchitobiose product analysis ?
chitin + H2O
-
 Homo sapiens ?
-
 r
additional information AMCase is important in cytokine Th2-mediated inflammation and is implicated in asthma and allergic disease, inhibition of AMCase results in increased airway inflammation and airway hyperresponsiveness, the enzyme's transglycosylation activity probably is a mechanism in vivo for feedback regulation of the chitinase activity Homo sapiens ?
-
 ?
additional information substrate specificity, overview, AMCase also shows low intrinsic transglycosylation activity, product analysis Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
acidic mammalian chitinase
-
 Homo sapiens
AMCase
-
 Homo sapiens
More AMCase is a glyco_18_domain-containing protein and a member of glycoside hydrolase family 18 Homo sapiens
TSA1902
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4 5 recombinant AMCAse Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
2 8 recombinant AMCase is inactive below pH 2.0 and above pH 8.0 Homo sapiens