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Literature summary for 3.2.1.14 extracted from

  • Ike, M.; Nagamatsu, K.; Shioya, A.; Nogawa, M.; Ogasawara, W.; Okada, H.; Morikawa, Y.
    Purification, characterization, and gene cloning of 46 kDa chitinase (Chi46) from Trichoderma reesei PC-3-7 and its expression in Escherichia coli (2006), Appl. Microbiol. Biotechnol., 71, 294-303.
    View publication on PubMed
Search for more literature for 3.2.1.14

Cloned(Commentary)

Cloned (Comment) Organism
gene chi46, DNA and amino acid sequence determination and analysis, multiple regulatory proteins are involved in wild-type Chi46 expression, expression in Escherichia coli Trichoderma reesei

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular the enzyme is secreted to the culture medium, the enzyme sequence contains a pre-pro type signal peptide Trichoderma reesei
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42265
-
 x * 46000, SDS-PAGE, x * 42265, Chi46, amino acid sequence calculation Trichoderma reesei
46000
-
 x * 46000, SDS-PAGE, x * 42265, Chi46, amino acid sequence calculation Trichoderma reesei

Organism

Organism UniProt Comment Textmining
Trichoderma reesei
-
 gene chi46
-
Trichoderma reesei PC-3-7
-
 gene chi46
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme from the culture medium Trichoderma reesei

Reaction

Reaction Comment Organism Reaction ID
Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins exo-type substrate cleavage mechanism Trichoderma reesei
a

Source Tissue

Source Tissue Comment Organism Textmining
mycelium
-
 Trichoderma reesei
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chitin + H2O colloidal and ethylene glycol chitin, exo-type cleavage mode Trichoderma reesei GlcNAc2 + ? main product from insoluble colloidal chitin ?
chitin + H2O colloidal and ethylene glycol chitin, exo-type cleavage mode Trichoderma reesei PC-3-7 GlcNAc2 + ? main product from insoluble colloidal chitin ?
chitosan 7B + H2O the activity increases with increasing acetylation of the substrate Trichoderma reesei ?
-
 ?
chitosan 7B + H2O the activity increases with increasing acetylation of the substrate Trichoderma reesei PC-3-7 ?
-
 ?
additional information multiple regulatory proteins are involved in Chi46 expression Trichoderma reesei ?
-
 ?
additional information multiple regulatory proteins are involved in Chi46 expression Trichoderma reesei PC-3-7 ?
-
 ?
N-acetylchito-oligosaccharides + H2O endo-type cleavage mode Trichoderma reesei ?
-
 ?
N-acetylchito-oligosaccharides + H2O endo-type cleavage mode Trichoderma reesei PC-3-7 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 46000, SDS-PAGE, x * 42265, Chi46, amino acid sequence calculation Trichoderma reesei
additional information the enzyme sequence contains a pre-pro type signal peptide Trichoderma reesei

Synonyms

Synonyms Comment Organism
Chi46
-
 Trichoderma reesei