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Literature summary for 3.2.1.14 extracted from
- Purification and characterization of an antimicrobial chitinase extracellularly produced by Monascus purpureus CCRC31499 in a shrimp and crab shell powder medium (2002), J. Agric. Food Chem., 50, 2249-2255.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| acetone | - |
Monascus purpureus |  |
| Hg2+ | - |
Monascus purpureus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Monascus purpureus | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | - |
Monascus purpureus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 81000 | - |
x * 81000, SDS-PAGE | Monascus purpureus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Monascus purpureus | - |
CCRC31499 | - |
| Monascus purpureus CCRC 31499 | - |
CCRC31499 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Monascus purpureus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.85 | - |
with ethylene glycol chitin as substrate | Monascus purpureus |
| 1.02 | - |
after Sephacryl S-200 purification step with colloidal chitin as substrate | Monascus purpureus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| colloidal chitin + H2O | - |
Monascus purpureus | ? | - |
? | |
| colloidal chitin + H2O | - |
Monascus purpureus CCRC 31499 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 81000, SDS-PAGE | Monascus purpureus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Monascus purpureus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 100 | - |
after 2 min loss of more than 40% enzyme activity | Monascus purpureus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Monascus purpureus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 8 | - |
Monascus purpureus |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Monascus purpureus | chromatofocusing | - |
4.9 |
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