Cloned (Comment) | Organism |
---|---|
expression in Hi-5 insect cells using baculovirus vectors | Manduca sexta |
Protein Variants | Comment | Organism |
---|---|---|
DELTA1-376 | ChiLCH, with a C-terminal His tag | Manduca sexta |
DELTA1-477 | ChiCH, with a C-terminal His tag | Manduca sexta |
DELTA1-79 | Chi80-535, enzymatically inactive | Manduca sexta |
DELTA377-535 | Chi376, no secretion into the medium | Manduca sexta |
DELTA387-477 | Chi386(MCBD) | Manduca sexta |
DELTA387-535 | Chi386 | Manduca sexta |
DELTA397-535 | Chi396 | Manduca sexta |
DELTA408-535 | Chi407 | Manduca sexta |
DELTA478-535 | Chi477 | Manduca sexta |
additional information | Chi386(MCBD)2: Chi386 protein fused with doubbled amino acid residues 478-535. Chi386(RCBD): Chi386 protein fused with amino acid residues 19-68 of a rice class I chitinase. ChiLH: linker region consisting of amino acid residues 377-477 with a C-terminal His tag, no secretion into the medium | Manduca sexta |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | the KM-values with chitin as substrate are for wild-type: 4.29 mg/ml, Chi477: 9.96 mg/ml, Chi396: 20 mg/ml, Chi386: 29 mg/ml, Chi386(MCBD): 4.98 mg/ml, Chi386(MCBD)2: 4.67 mg/ml and Chi386(RCBD): 3.18 mg/ml | Manduca sexta | |
0.29 | - |
4-methylumbelliferyl beta-N,N',N''-triacetylchitotrioside | 37°C, pH 6, Chi386(MCBD) | Manduca sexta | |
0.31 | - |
4-methylumbelliferyl beta-N,N',N''-triacetylchitotrioside | 37°C, pH 6, Chi386(MCBD)2 | Manduca sexta | |
0.48 | - |
4-methylumbelliferyl beta-N,N',N''-triacetylchitotrioside | 37°C, pH 6, Chi386(RCBD) | Manduca sexta | |
0.51 | - |
4-methylumbelliferyl beta-N,N',N''-triacetylchitotrioside | 37°C, pH 6, Chi477 | Manduca sexta | |
0.64 | - |
4-methylumbelliferyl beta-N,N',N''-triacetylchitotrioside | 37°C, pH 6, Chi396 | Manduca sexta | |
0.93 | - |
4-methylumbelliferyl beta-N,N',N''-triacetylchitotrioside | 37°C, pH 6, Chi386 | Manduca sexta | |
1.13 | - |
4-methylumbelliferyl beta-N,N',N''-triacetylchitotrioside | 37°C, pH 6, wild-type | Manduca sexta |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
79000 | - |
x * 79000, SDS-PAGE | Manduca sexta |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Manduca sexta | - |
tobacco hornworm | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | glycosylated with mannose and N-acetylglucosamine as most abundant sugars, glucose and galactose | Manduca sexta |
Purification (Comment) | Organism |
---|---|
- |
Manduca sexta |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-methylumbelliferyl beta-N,N',N''-triacetylchitotrioside + H2O | - |
Manduca sexta | ? | - |
? | |
chitin + H2O | - |
Manduca sexta | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 79000, SDS-PAGE | Manduca sexta |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.14 | - |
4-methylumbelliferyl beta-N,N',N''-triacetylchitotrioside | 37°C, pH 6, Chi386(MCBD) | Manduca sexta | |
0.17 | - |
4-methylumbelliferyl beta-N,N',N''-triacetylchitotrioside | 37°C, pH 6, Chi386(MCBD)2 | Manduca sexta | |
0.22 | - |
4-methylumbelliferyl beta-N,N',N''-triacetylchitotrioside | 37°C, pH 6, Chi386(RCBD) | Manduca sexta | |
0.27 | - |
4-methylumbelliferyl beta-N,N',N''-triacetylchitotrioside | 37°C, pH 6, Chi396 | Manduca sexta | |
0.31 | - |
4-methylumbelliferyl beta-N,N',N''-triacetylchitotrioside | 37°C, pH 6, Chi477 | Manduca sexta | |
0.37 | - |
4-methylumbelliferyl beta-N,N',N''-triacetylchitotrioside | 37°C, pH 6, Chi386 | Manduca sexta | |
0.76 | - |
4-methylumbelliferyl beta-N,N',N''-triacetylchitotrioside | 37°C, pH 6, wild-type | Manduca sexta |