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Literature summary for 3.2.1.14 extracted from

  • Kaomek, M.; Mizuno, K.; Fujimura, T.; Sriyotha, P.; Cairns, J.R.
    Cloning, expression, and characterization of an antifungal chitinase from Leucaena leucocephala de Wit (2003), Biosci. Biotechnol. Biochem., 67, 667-676.
    View publication on PubMed
Search for more literature for 3.2.1.14

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli containing a N-terminal additional Met and a C-terminal His tag Leucaena leucocephala

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32000
-
 x * 32000, SDS-PAGE Leucaena leucocephala

Organism

Organism UniProt Comment Textmining
Leucaena leucocephala
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Leucaena leucocephala

Source Tissue

Source Tissue Comment Organism Textmining
seed
-
 Leucaena leucocephala
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.057
-
 purified recombinant chitinase Leucaena leucocephala

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chitin + H2O colloidal, purified and swollen chitin Leucaena leucocephala ?
-
 ?
chitosan
-
 Leucaena leucocephala ?
-
 ?
glycol chitin + H2O
-
 Leucaena leucocephala ?
-
 ?
p-nitrophenyl-beta-D-N,N',N''-triacetylchitotriose + H2O
-
 Leucaena leucocephala N,N',N''-triacetylchitotriose + p-nitrophenol
-
 ?

Subunits

Subunits Comment Organism
? x * 32000, SDS-PAGE Leucaena leucocephala

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
 Leucaena leucocephala

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
-
 Leucaena leucocephala

pI Value

Organism Comment pI Value Maximum pI Value
Leucaena leucocephala isoelectric focusing
-
 7.5