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Literature summary for 3.2.1.14 extracted from

  • Truong, N.H.; Park, S.M.; Nishizawa, Y.; Watanabe, T.; Sasaki, T.; Itoh, Y.
    Structure, heterologous expression, and properties of rice (Oryza sativa L.) family 19 chitinases (2003), Biosci. Biotechnol. Biochem., 67, 1063-1070.
    View publication on PubMed
Search for more literature for 3.2.1.14

Cloned(Commentary)

Cloned (Comment) Organism
expression in Pichia pastoris Oryza sativa

Protein Variants

Protein Variants Comment Organism
OsChia1cDELTACBD deletion of chitosine-binding domain Oryza sativa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information OsChia1c: 0.9 mg/ml with colloidal chitin as substrate and 0.4 mg/ml with glycol chitin as substrate, OsChia1cDELTACBD: 1.2 mg/ml with colloidal chitin as substrate and 0.2 mg/ml with glycol chitin as substrate, OsChia2b: 1.9 mg/ml with colloidal chitin as substrate and 0.4 mg/ml with glycol chitin as substrate Oryza sativa

Organism

Organism UniProt Comment Textmining
Oryza sativa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Oryza sativa

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.9
-
 OsChia1cDELTACBD with colloidal chitin as substrate Oryza sativa
2.1
-
 OsChia1c with colloidal chitin as substrate Oryza sativa
2.3
-
 OsChia2b with colloidal chitin as substrate Oryza sativa
52
-
 OsChia1c with colloidal glycol as substrate Oryza sativa
83
-
 OsChia1cDELTACBD with glycol chitin as substrate Oryza sativa
116
-
 OsChia2b with glycol chitin as substrate Oryza sativa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chitin + H2O colloidal Oryza sativa ?
-
 ?
glycol chitin + H2O
-
 Oryza sativa ?
-
 ?
N,N',N'',N''',N'''',N'''''-hexaacetylchitohexaose + H2O
-
 Oryza sativa N,N'-diacetylchitobiose + N,N',N'',N'''-tetraacetylchitotetraose + N,N',N''-triacetylchitotriose OsChia1c almost exclusively generates (GlcNAc)3 and small amounts of (GlcNAc)2 and (GlcNAc)4, OsChia2b hydrolyzes the substrate to predominantely yield (GlcNAc)2 ?
N,N',N'',N''',N''''-pentaacetylchitopentaose + H2O poorly hydrolyzed by OsChia1c but efficiently hydrolyzed by OsChia2b to predominantely yield (GlcNAc)2 Oryza sativa N,N'-diacetylchitobiose + N,N',N''-triacetylchitotriose
-
 ?
N,N',N'',N'''-tetraacetylchitotetraose + H2O poorly hydrolyzed by OsChia1c but efficiently hydrolyzed by OsChia2b to predominantely yield (GlcNAc)2 Oryza sativa 2 N,N'-diacetylchitobiose
-
 ?
N,N',N''-triacetylchitotriose + H2O OsChia2b Oryza sativa N-acetyl-D-glucosamine + N,N'-diacetylchitobiose
-
 ?

Synonyms

Synonyms Comment Organism
OsChia1c
-
 Oryza sativa
OsChia1d
-
 Oryza sativa
OsChia2a
-
 Oryza sativa
OsChia2b
-
 Oryza sativa
OsChia4a
-
 Oryza sativa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
-
 Oryza sativa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.8
-
 OsChia1c and OsChia1cDELTACBD Oryza sativa
5.2
-
 OsChia2b Oryza sativa