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Literature summary for 3.2.1.14 extracted from
- Purification and characterization of two chitinases form the leaves of pokeweed (Phytolacca americana) (1995), Biosci. Biotechnol. Biochem., 59, 656-661.
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 25000 | - |
x * 25000, SDS-PAGE in presence of beta-mercaptoethanol, PLC-A,x * 29000, SDS-PAGE in presence of beta-mercaptoethanol, PLC-B | Phytolacca americana |
| 29000 | - |
x * 25000, SDS-PAGE in presence of beta-mercaptoethanol, PLC-A,x * 29000, SDS-PAGE in presence of beta-mercaptoethanol, PLC-B | Phytolacca americana |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Phytolacca americana | - |
pokeweed | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| both isoenzymes purified to homogeneity | Phytolacca americana |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1120 | - |
isoenzyme PLC-B | Phytolacca americana |
| 1546 | - |
isoenzyme PLC-A | Phytolacca americana |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cell wall + H2O | PLC-B hydrolyses Micrococcus lysodeikticus cell wall | Phytolacca americana | chitin oligosaccharides | - |
? |  |
| chitin + H2O | colloidal chitin | Phytolacca americana | N,N-diacetylchitobiose + N-acetylglucosamine | - |
? | |
| chitohexaose + H2O | PLC-A and PLC-B | Phytolacca americana | chitobiose + chitotriose | PLC-B N-acetylglucosamine, chitobiose and chitotetraose only | ? | |
| chitopentaose + H2O | PLC-A, PLC-B | Phytolacca americana | chitobiose + chitotriose | PLC-B chitotetraose and N-acetylglucosamine only | ? | |
| chitotetraose + H2O | PLC-A | Phytolacca americana | N-acetylglucosamine + chitobiose + chitotriose | chitobiose only | ? | |
| glycol chitin + H2O | - |
Phytolacca americana | N-acetylglucosamine + ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 25000, SDS-PAGE in presence of beta-mercaptoethanol, PLC-A,x * 29000, SDS-PAGE in presence of beta-mercaptoethanol, PLC-B | Phytolacca americana |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PLC-A | - |
Phytolacca americana |
| PLC-B | - |
Phytolacca americana |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
stable below 40°C | Phytolacca americana |
| 70 | - |
PLC-B completely inactivated | Phytolacca americana |
| 80 | - |
PLC-A completely inactivated | Phytolacca americana |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 3 | - |
PLC-B, also active peak at pH 7 | Phytolacca americana |
| 4.5 | - |
PLC-A | Phytolacca americana |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 8 | PLC-B is more stable than PLC-A | Phytolacca americana |
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