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Literature summary for 3.2.1.14 extracted from
- Purification, characterization and differential hormonal regulation of a beta-1,3-glucanase and two chitinases from chickpea (Cicer arietinum L.) (1993), Planta, 189, 60-69.
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Cicer arietinum | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 28000 | - |
x * 28000, acidic chitinase, SDS-PAGE | Cicer arietinum |
| 32000 | - |
x * 32000, basic chitinase, SDS-PAGE | Cicer arietinum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cicer arietinum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| acidic chitinase, 7.5fold, basic chitinase, 19fold | Cicer arietinum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture filtrate | - |
Cicer arietinum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Cicer arietinum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| carboxymethylated-chitin-remazol brilliant violet + H2O | - |
Cicer arietinum | ? | - |
? |  |
| chitin + H2O | - |
Cicer arietinum | N,N-diacetylchitobiose + N-acetylglucosamine | basic chitinase produces chitobiose and chitotriose, acidic chitinase produces chitobiose and chitotetraose | ? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 32000, basic chitinase, SDS-PAGE | Cicer arietinum |
| ? | x * 28000, acidic chitinase, SDS-PAGE | Cicer arietinum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
basic chitinase | Cicer arietinum |
| 70 | - |
acidic chitinase | Cicer arietinum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4 | - |
acidic chitinase | Cicer arietinum |
| 7 | - |
basic chitinase | Cicer arietinum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3.2 | 5.5 | acidic chitinase | Cicer arietinum |
| 4.9 | 8.8 | basic chitinase | Cicer arietinum |
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Release 2023.1 (January 2023)
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