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Literature summary for 3.2.1.14 extracted from
- Chitinase in goat serum. Preliminary purification and characterization (1974), Eur. J. Biochem., 46, 367-376.
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 59000 | 60000 | gel filtration | Capra hircus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
| Capra hircus | - |
- |
- |
| Gallus gallus | - |
- |
- |
| Mus musculus | - |
- |
- |
| Ovis aries | - |
- |
- |
| Rattus norvegicus | - |
- |
- |
| Sus scrofa | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 100fold | Capra hircus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| abomasum | - |
Capra hircus | - |
| abomasum | mucosa, mucus and wall | Bos taurus | - |
| kidney | - |
Bos taurus | - |
| leukocyte | - |
Bos taurus | - |
| leukocyte | - |
Capra hircus | - |
| pancreas | - |
Bos taurus | - |
| pancreas | - |
Capra hircus | - |
| serum | - |
Gallus gallus | - |
| serum | - |
Mus musculus | - |
| serum | - |
Rattus norvegicus | - |
| serum | - |
Sus scrofa | - |
| serum | - |
Bos taurus | - |
| serum | - |
Ovis aries | - |
| serum | - |
Capra hircus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Bos taurus |
| additional information | - |
2870 Hultin Units/mg | Capra hircus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 3 weeks, 84% activity loss | Capra hircus |
| 25°C, 3 weeks, no loss of activity | Capra hircus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| chitin + H2O | - |
Bos taurus | N,N-diacetylchitobiose + N-acetylglucosamine | - |
? |  |
| chitin + H2O | - |
Capra hircus | N,N-diacetylchitobiose + N-acetylglucosamine | - |
? | |
| glycol chitin + H2O | - |
Bos taurus | N-acetylglucosamine + ? | - |
? | |
| glycol chitin + H2O | - |
Capra hircus | N-acetylglucosamine + ? | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Bos taurus |
| 60 | - |
50% activity loss after 60 min | Capra hircus |
| 65 | - |
- |
Bos taurus |
| 65 | - |
complete inactivation after 60 min | Capra hircus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 1.65 | - |
glycol chitin | Capra hircus |
| 5.5 | - |
colloidal chitin | Capra hircus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 7 | colloidal chitin as substrate | Capra hircus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 2.5 | 8 | - |
Capra hircus |
| 5.5 | - |
maximum stabtility at pH 5.5 | Capra hircus |
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