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Literature summary for 3.2.1.14 extracted from

  • Roberts, R.L.; Cabib, E.
    Serratia marcescens chitinase: one-step purification and use for the determination of chitin (1982), Anal. Biochem., 127, 402-412.
    View publication on PubMed
Search for more literature for 3.2.1.14

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.4
-
 chitin expressed in N-acetylglucosamine equivalents Serratia marcescens

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Serratia marcescens
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
52000
-
 x * 52000 + x * 58000, SDS-PAGE Serratia marcescens
58000
-
 x * 52000 + x * 58000, SDS-PAGE Serratia marcescens

Organism

Organism UniProt Comment Textmining
Serratia marcescens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
5.9fold Serratia marcescens

Source Tissue

Source Tissue Comment Organism Textmining
culture filtrate
-
 Serratia marcescens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.33
-
-
 Serratia marcescens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cell wall + H2O from yeast Serratia marcescens chitin oligosaccharides N-acetetylglucosamine and diacetylchitobiose ?
chitin + H2O from ground crab shell Serratia marcescens N,N-diacetylchitobiose + N-acetylglucosamine with high ratio of chitinase to chitin N-acetylglucosamine only ?
chitin + H2O [acetyl-3H]chitin Serratia marcescens N,N-diacetylchitobiose + N-acetylglucosamine with high ratio of chitinase to chitin N-acetylglucosamine only ?

Subunits

Subunits Comment Organism
? x * 52000 + x * 58000, SDS-PAGE Serratia marcescens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4 7
-
 Serratia marcescens