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Literature summary for 3.2.1.14 extracted from

  • Tsukamoto, T.; Koga, D.; Ide, A.; Ishibashi, T.; Horino-Matsushige, M.; Yagishita, K.; Imoto, T.
    Purification and some properties of chitinases from yam, Dioscorea opposita THUMB (1984), Agric. Biol. Chem., 48, 931-939.
No PubMed abstract available
Search for more literature for 3.2.1.14

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33500
-
 x * 33500, SDS-PAGE, isoenzymes I-III Dioscorea oppositifolia

Organism

Organism UniProt Comment Textmining
Dioscorea oppositifolia
-
-
-

Purification (Commentary)

Purification (Comment) Organism
isoenzyme I, 27.4fold, isoenzyme II, 14.1fold, isoenzyme III 39.7fold Dioscorea oppositifolia

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Dioscorea oppositifolia

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glycol chitin + H2O
-
 Dioscorea oppositifolia N-acetylglucosamine + ?
-
 ?

Subunits

Subunits Comment Organism
? x * 33500, SDS-PAGE, isoenzymes I-III Dioscorea oppositifolia

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 stable below 60°C, isoenzymes I and III Dioscorea oppositifolia

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5
-
 isoenzyme I and III, first optimum Dioscorea oppositifolia
8
-
 isoenzyme III, second optimum Dioscorea oppositifolia
8.5
-
 isoenzyme I, second optimum Dioscorea oppositifolia

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 11 isoenzymes I and III Dioscorea oppositifolia