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Literature summary for 3.2.1.14 extracted from

  • Boller, T.; Gehri, A.; Mauch, F.; Vögeli, U.
    Chitinase from Phaseolus vulgaris leaves (1988), Methods Enzymol., 161, 479-484.
No PubMed abstract available
Search for more literature for 3.2.1.14

Inhibitors

Inhibitors Comment Organism Structure
histamine
-
 Phaseolus vulgaris
N-acetylglucosamine less than 10% inhibition Phaseolus vulgaris

Localization

Localization Comment Organism GeneOntology No. Textmining
vacuole central Phaseolus vulgaris 5773
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
22000
-
 gel filtration Phaseolus vulgaris
30500
-
 ultracentrifugation Phaseolus vulgaris
32500
-
 SDS-PAGE Phaseolus vulgaris

Organism

Organism UniProt Comment Textmining
Phaseolus vulgaris
-
-
-

Purification (Commentary)

Purification (Comment) Organism
24fold Phaseolus vulgaris

Source Tissue

Source Tissue Comment Organism Textmining
leaf primary leaves Phaseolus vulgaris
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
144
-
-
 Phaseolus vulgaris

Storage Stability

Storage Stability Organism
-20°C, 2 years, no loss of activity Phaseolus vulgaris

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chitin + H2O [acetyl-3H]chitin Phaseolus vulgaris N,N-diacetylchitobiose + N-acetylglucosamine
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 stable below 40°C Phaseolus vulgaris

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
 Phaseolus vulgaris

pH Range

pH Minimum pH Maximum Comment Organism
3 9
-
 Phaseolus vulgaris