Literature summary for 3.2.1.136 extracted from

Urbanikova, L.; Vrsanska, M.; Morkeberg Krogh, K.B.; Hoff, T.; Biely, P.
Structural basis for substrate recognition by Erwinia chrysanthemi GH30 glucuronoxylanase (2011), FEBS J., 278, 2105-2116.

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Bacillus subtilis strain A164delta5	Dickeya chrysanthemi

Crystallization (Commentary)

Crystallization (Comment)	Organism
in complex with beta-D-xylopyranosyl-(1->4)-[4-O-methyl-alpha-D-glucuronosyl-(1->2)]-beta-D-xylopyranosyl-(1->4)-D-xylose,hanging drop vapor diffusion method, using 0.1 M imidazole/D,L-malic acid buffer (pH 7.5) and 20% (w/v) poly(ethylene glycol) 1500	Dickeya chrysanthemi

Organism

Organism	UniProt	Comment	Textmining
Dickeya chrysanthemi	Q46961	-	-

Purification (Commentary)

Purification (Comment)	Organism
MEP HyperCel column chromatography, SP Sepharose column chromatography, and XK 26/20 column chromatography	Dickeya chrysanthemi

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-O-methyl-alpha-D-glucuronosyl-(1->2)-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylose + H2O	-	Dickeya chrysanthemi	?	-	?
beta-D-xylopyranosyl-(1->4)-[4-O-methyl-alpha-D-glucuronosyl-(1->2)]-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylose + h2O	-	Dickeya chrysanthemi	?	-	?
glucuronoxylan + H2O	the cleavage of the glucuronoxylan main chain takes place exclusively at the second glycosidic linkage from the branch towards the reducing end of the polysaccharide chain	Dickeya chrysanthemi	?	-	?

Synonyms

Synonyms	Comment	Organism
glucuronoxylanase	-	Dickeya chrysanthemi
XynA	-	Dickeya chrysanthemi

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Release 2023.1 (January 2023)