Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.135 extracted from

  • Ece, S.; Evran, S.; Janda, J.O.; Merkl, R.; Sterner, R.
    Improving thermal and detergent stability of Bacillus stearothermophilus neopullulanase by rational enzyme design (2015), Protein Eng. Des. Sel., 28, 147-151 .
    View publication on PubMed
Search for more literature for 3.2.1.135

Application

Application Comment Organism
industry potentially valuable enzyme for starch and detergent industries Geobacillus stearothermophilus

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli CodonPlus BL21 (DE3) Geobacillus stearothermophilus

Protein Variants

Protein Variants Comment Organism
A416I t1/2 of mutant enzyme at 70°C is 17 min, compared to 15 min for wild-type enzyme. Mutation does not compromise the catalytic activity Geobacillus stearothermophilus
A566L t1/2 of mutant enzyme at 70°C is 27 min, compared to 15 min for wild-type enzyme. Mutation does not compromise the catalytic activity Geobacillus stearothermophilus
D46E t1/2 of mutant enzyme at 70°C is 100 min, compared to 15 min for wild-type enzyme. Mutation does not compromise the catalytic activity Geobacillus stearothermophilus
N413Q t1/2 of mutant enzyme at 70°C is 32 min, compared to 15 min for wild-type enzyme. Mutation does not compromise the catalytic activity Geobacillus stearothermophilus
S407T t1/2 of mutant enzyme at 70°C is 66 min, compared to 15 min for wild-type enzyme. Mutation does not compromise the catalytic activity Geobacillus stearothermophilus
V239L t1/2 of mutant enzyme at 70°C is 103 min, compared to 15 min for wild-type enzyme. Mutation does not compromise the catalytic activity Geobacillus stearothermophilus
V374I t1/2 of mutant enzyme at 70°C is 14 min, compared to 15 min for wild-type enzyme. Mutation does not compromise the catalytic activity Geobacillus stearothermophilus
V404L t1/2 of mutant enzyme at 70°C is 191 min, compared to 15 min for wild-type enzyme. Mutation does not compromise the catalytic activity Geobacillus stearothermophilus
V533L t1/2 of mutant enzyme at 70°C is 8 min, compared to 15 min for wild-type enzyme. Mutation does not compromise the catalytic activity Geobacillus stearothermophilus

Organism

Organism UniProt Comment Textmining
Geobacillus stearothermophilus P38940
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Geobacillus stearothermophilus

Synonyms

Synonyms Comment Organism
bsNpl
-
 Geobacillus stearothermophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
 t1/2: 15 min (wild-type enzyme), 100 min (mutant enzyme D46E), 103 min (mutant enzyme V239L), 14 min (mutant enzyme V374I), 191 min (mutant enzyme V404L), 66 min (mutant enzyme S407T), 32 min (mutant enzyme N413Q), 17 min (mutant enzyme A416I), 8 min (mutant enzyme V533L), 27 min (mutant enzyme A566L) Geobacillus stearothermophilus