Cloned (Comment) | Organism |
---|---|
NCL21 with plasmid pRN-PulSa, pRN-Puldelta3 | Lactiplantibacillus plantarum |
Protein Variants | Comment | Organism |
---|---|---|
additional information | C-terminal truncated ApuA produced with Escherichia coli plasmid, and transformed into Lactobacillus plantarum | Lactiplantibacillus plantarum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
alpha-cyclodextrin | moderate inhibition by 10 mM, 30°C, 1 h | Lactiplantibacillus plantarum | |
gamma-cyclodextrin | moderate inhibition by 10 mM, 30°C, 1 h | Lactiplantibacillus plantarum | |
Guanidine-HCl | inhibition of both enzyme variants at 1 M, 30°C, 1 h | Lactiplantibacillus plantarum | |
additional information | no inhibition by 10 mM EDTA and beta-cyclodextrin, 30°C, 1 h | Lactiplantibacillus plantarum | |
N-bromosuccinimide | strong inhibition by 0.1 mM, 30°C, 1 h | Lactiplantibacillus plantarum | |
Urea | inhibition of both enzyme variants at 5 M, 30°C, 1 h | Lactiplantibacillus plantarum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | 2.6 g/l, amylose, full length enzyme, pH 4.5, 40°C | Lactiplantibacillus plantarum | |
additional information | - |
additional information | 2.6 g/l, pullulan, C-terminal truncated enzyme ApuADELTA, pH 4.5, 40°C | Lactiplantibacillus plantarum | |
additional information | - |
additional information | 3.3 g/l, amylose, C-terminal truncated enzyme ApuADELTA, pH 4.5, 40°C | Lactiplantibacillus plantarum | |
additional information | - |
additional information | 5.0 g/l, starch, C-terminal truncated enzyme ApuADELTA, pH 4.5, 40°C | Lactiplantibacillus plantarum | |
additional information | - |
additional information | 6.9 g/l, pullulan, full length enzyme, pH 4.5, 40°C | Lactiplantibacillus plantarum | |
additional information | - |
additional information | 7.7 g/l, starch, full length enzyme, pH 4.5, 40°C | Lactiplantibacillus plantarum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
CaCl2 | 1 mM C-terminal truncated enzyme ApuADELTA: amylase activity: 105%, pullulanase activity: 94% | Lactiplantibacillus plantarum | |
CaCl2 | 1 mM full length enzyme: amylase activity: 111%, pullulanase activity: 101% | Lactiplantibacillus plantarum | |
CoCl2 | 1 mM C-terminal truncated enzyme ApuADELTA: amylase activity: 128%, pullulanase activity: 130% | Lactiplantibacillus plantarum | |
CoCl2 | 1 mM full length enzyme: amylase activity: 150%, pullulanase activity: 140% | Lactiplantibacillus plantarum | |
KCl | 50 mM C-terminal truncated enzyme ApuADELTA: amylase activity: 106%, pullulanase activity: 93% | Lactiplantibacillus plantarum | |
KCl | 50 mM full length enzyme: amylase activity: 112%, pullulanase activity: 99% | Lactiplantibacillus plantarum | |
MnCl2 | 1 mM C-terminal truncated enzyme ApuADELTA: amylase activity: 109%, pullulanase activity: 121% | Lactiplantibacillus plantarum | |
MnCl2 | 1 mM full length enzyme: amylase activity: 133%, pullulanase activity: 133% | Lactiplantibacillus plantarum | |
NaCl | 50 mM C-terminal truncated enzyme ApuADELTA: amylase activity: 103%, pullulanase activity: 94% | Lactiplantibacillus plantarum | |
NaCl | 50 mM full length enzyme: amylase activity: 107%, pullulanase activity: 95% | Lactiplantibacillus plantarum | |
NiCl2 | 1 mM C-terminal truncated enzyme ApuADELTA: amylase activity: 87%, pullulanase activity: 100% | Lactiplantibacillus plantarum | |
NiCl2 | 1 mM full length enzyme: amylase activity: 116%, pullulanase activity: 112% | Lactiplantibacillus plantarum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lactiplantibacillus plantarum | - |
L137 | - |
Lactiplantibacillus plantarum ATCC BAA-793 | - |
L137 | - |
Purification (Comment) | Organism |
---|---|
supernatant ultrafiltrated, DEAE Sepharose CLl-4B, Superose 6 | Lactiplantibacillus plantarum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.00000431 | - |
full length enzyme | Lactiplantibacillus plantarum |
0.00000596 | - |
C-terminal truncated enzyme ApuADELTA | Lactiplantibacillus plantarum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
amylose + H2O | 61% larger oligosaccharides than maltoocataose after 60 min of C-terminal truncated enzyme ApuADELTA activity compared to 92.6% of full length enzyme activity | Lactiplantibacillus plantarum | maltotriose + maltotetraose + maltopentaose | no glucose, nor maltose | ? | |
amylose + H2O | 61% larger oligosaccharides than maltoocataose after 60 min of C-terminal truncated enzyme ApuADELTA activity compared to 92.6% of full length enzyme activity | Lactiplantibacillus plantarum ATCC BAA-793 | maltotriose + maltotetraose + maltopentaose | no glucose, nor maltose | ? | |
pullulan + H2O | no larger oligosaccharides than maltoocataose after 5 min of C-terminal truncated enzyme ApuADELTA activity compared to 95.5% of ApuA activity | Lactiplantibacillus plantarum | maltotriose + maltopentaose + maltohexaose + maltoheptaose | no glucose, nor maltose | ? | |
pullulan + H2O | no larger oligosaccharides than maltoocataose after 5 min of C-terminal truncated enzyme ApuADELTA activity compared to 95.5% of ApuA activity | Lactiplantibacillus plantarum ATCC BAA-793 | maltotriose + maltopentaose + maltohexaose + maltoheptaose | no glucose, nor maltose | ? | |
soluble starch + H2O | - |
Lactiplantibacillus plantarum | maltotriose + maltotetraose | no glucose, nor maltose | ? | |
soluble starch + H2O | - |
Lactiplantibacillus plantarum ATCC BAA-793 | maltotriose + maltotetraose | no glucose, nor maltose | ? |
Synonyms | Comment | Organism |
---|---|---|
amylopullalanase | - |
Lactiplantibacillus plantarum |
ApuA | full length enzyme | Lactiplantibacillus plantarum |
ApuADELTA | C-terminal truncated enzyme | Lactiplantibacillus plantarum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
40°C and 45°C with about 100% activity for full length enzyme and C-terminal truncated enzyme ApuADELTA, pH 4, starch as substrate | Lactiplantibacillus plantarum |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 60 | C-terminal truncated enzyme ApuADELTA with only slightly higher activities than full length enzyme, especially at higher than optimal temperatures, about 100% activity at 40-45°C, 60-90% activity at 20-35°C and 50°C, 20-40% activity at 55-60°C, 0.1 M sodium acetate butter, pH 4.5, starch as substrate | Lactiplantibacillus plantarum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 60 | C-terminal truncated enzyme ApuADELTA, 100% activity at 20-30°C, 50% at 45°C, pre-incubation at given temperatures for 1 h, pH 4.5, residual activity measured at 40°C, starch as substrate | Lactiplantibacillus plantarum |
20 | 60 | full length enzyme, 100% activity at 20-30°C, 50% activity at 41°C, pre-incubation at given temperatures for 1 h, pH 4.5, residual activity measured at 40°C, starch as substrate | Lactiplantibacillus plantarum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | - |
full length enzyme and C-terminal truncated enzyme ApuADELTA with optimum at pH 4-4.5, 0.1 M buffers pH 2.5-4: glycine-HCl, pH 3.5-6: acetate, pH 6-8: phosphate, substrate: soluble starch | Lactiplantibacillus plantarum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
2 | 7 | 40°C, 0.1 M buffers pH 2.5-4: glycine-HCl, pH 3.5-6: acetate, pH 6-8: phosphate, substrate: soluble starch | Lactiplantibacillus plantarum |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
2 | 8 | C-terminal truncated enzyme ApuADELTA, pre-incubation for 1 h, 40°C, 0.1 M buffers pH 2.5-8, 90-100% activity from pH 2.5-6.6, about 80% activity at pH 7, about 60% at pH 7.5, about 30% activity at pH 8 | Lactiplantibacillus plantarum |
2 | 8 | full length enzyme slightly less stable than C-terminal truncated enzyme ApuADELTA, pre-incubation for 1 h, 40°C, 0.1 M buffers pH 2.5-8, 90-100% activity from pH 2.5-6, about 80% activity at pH 6.5, about 50% at pH 7, between 20-30% activity at pH 7.5 and 8 | Lactiplantibacillus plantarum |