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Literature summary for 3.2.1.135 extracted from

  • Cheong, K.A.; Kim, T.J.; Yoon, J.W.; Park, C.S.; Lee, T.S.; Kim, Y.B.; Park, K.H.; Kim, J.W.
    Catalytic activities of intracellular dimeric neopullulanase on cyclodextrin, acarbose and maltose (2002), Biotechnol. Appl. Biochem., 35, 27-34.
    View publication on PubMed
Search for more literature for 3.2.1.135

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Geobacillus stearothermophilus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
65000
-
 enzyme likely to be present in monomer-dimer equilibrium with a molecular ratio of 1:9 in 50 mM sodium acetate buffer, pH 6.0, x * 65000, SDS-PAGE, x * 70156, MALDI-TOF MS analyzis Geobacillus stearothermophilus
70156
-
 enzyme likely to be present in monomer-dimer equilibrium with a molecular ratio of 1:9 in 50 mM sodium acetate buffer, pH 6.0, x * 65000, SDS-PAGE, x * 70156, MALDI-TOF MS analyzis Geobacillus stearothermophilus
140000
-
 gel filtration Geobacillus stearothermophilus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
pullulan + H2O Geobacillus stearothermophilus
-
 panose + ?
-
 ?
pullulan + H2O Geobacillus stearothermophilus IMA6503
-
 panose + ?
-
 ?

Organism

Organism UniProt Comment Textmining
Geobacillus stearothermophilus Q9AIV2 IMA6503
-
Geobacillus stearothermophilus IMA6503 Q9AIV2 IMA6503
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Geobacillus stearothermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acarbose + H2O
-
 Geobacillus stearothermophilus pseudotrisaccharide + D-glucose and transglycosylation of D-glucose to isoacarbose ?
acarbose + H2O
-
 Geobacillus stearothermophilus IMA6503 pseudotrisaccharide + D-glucose and transglycosylation of D-glucose to isoacarbose ?
beta-cyclomaltodextrin + H2O
-
 Geobacillus stearothermophilus maltose + D-glucose
-
 ?
beta-cyclomaltodextrin + H2O
-
 Geobacillus stearothermophilus IMA6503 maltose + D-glucose
-
 ?
additional information the enzyme catalyses condensation between two maltose molecules and subsequent hydrolysis of the resulting 6-O-alpha-maltosylmaltose to D-glucose and panose, when maltose concentration is inceased to 20% Geobacillus stearothermophilus ?
-
 ?
additional information enzyme hydrolyzes starch Geobacillus stearothermophilus ?
-
 ?
additional information the enzyme catalyses condensation between two maltose molecules and subsequent hydrolysis of the resulting 6-O-alpha-maltosylmaltose to D-glucose and panose, when maltose concentration is inceased to 20% Geobacillus stearothermophilus IMA6503 ?
-
 ?
additional information enzyme hydrolyzes starch Geobacillus stearothermophilus IMA6503 ?
-
 ?
pullulan + H2O
-
 Geobacillus stearothermophilus panose + ?
-
 ?
pullulan + H2O
-
 Geobacillus stearothermophilus IMA6503 panose + ?
-
 ?

Subunits

Subunits Comment Organism
dimer
-
 Geobacillus stearothermophilus
monomer
-
 Geobacillus stearothermophilus
More enzyme likely to be present in monomer-dimer equilibrium with a molecular ratio of 1:9 in 50 mM sodium acetate buffer, pH 6.0, x * 65000, SDS-PAGE, x * 70156, MALDI-TOF MS analyzis Geobacillus stearothermophilus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
 Geobacillus stearothermophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Geobacillus stearothermophilus