Application | Comment | Organism |
---|---|---|
synthesis | panose migth be used as an anticariogenic sweetener in foods, development of a system for continuous production of extremely high panose syrup from pullulan by employing the enzyme | Bacteroides thetaiotaomicron |
Protein Variants | Comment | Organism |
---|---|---|
additional information | diverse mutants are constructed and specific activities, sugar compositions of pullulan hydrolysate and hydrolysis activities toward alpha-(1-4) and alpha-(1-6)-glucosidic linkages analyzed | Bacteroides thetaiotaomicron |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
70000 | - |
gel filtration | Bacteroides thetaiotaomicron |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
pullulan + H2O | Bacteroides thetaiotaomicron | - |
panose + ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacteroides thetaiotaomicron | - |
95-1 | - |
Bacteroides thetaiotaomicron 95-1 | - |
95-1 | - |
Purification (Comment) | Organism |
---|---|
- |
Bacteroides thetaiotaomicron |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
comparison of wild-type and mutant enzymes | Bacteroides thetaiotaomicron |
Storage Stability | Organism |
---|---|
-20°C, 20% glycerol, stable | Bacteroides thetaiotaomicron |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
maltoheptaose + H2O | - |
Bacteroides thetaiotaomicron | D-glucose + maltose + ? | - |
? | |
maltohexaose + H2O | - |
Bacteroides thetaiotaomicron | D-glucose + maltose + ? | - |
? | |
maltopentaose + H2O | - |
Bacteroides thetaiotaomicron | D-glucose + maltose + ? | - |
? | |
maltotetraose + H2O | - |
Bacteroides thetaiotaomicron | 2 D-glucose + maltose | - |
? | |
maltotriose + H2O | - |
Bacteroides thetaiotaomicron | maltose + D-glucose | - |
? | |
additional information | enzyme is able to cleave beta-cyclodextrin | Bacteroides thetaiotaomicron | ? | - |
? | |
additional information | enzyme also cleaves alpha-1,4-bonds in amylose and in oligosaccharides of maltotriose through maltoheptaose in chain length | Bacteroides thetaiotaomicron | ? | - |
? | |
pullulan + H2O | - |
Bacteroides thetaiotaomicron | panose + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 70000, SDS-PAGE | Bacteroides thetaiotaomicron |