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Literature summary for 3.2.1.133 extracted from

  • Sulong, M.; Leow, T.; Rahman, R.; Basri, M.; Salleh, A.
    Characteristics of recombinant maltogenic amylase from Geobacillus sp. SK70 (2017), Indian J. Biotechnol., 16, 91-99 .
No PubMed abstract available
Search for more literature for 3.2.1.133

Activating Compound

Activating Compound Comment Organism Structure
Tween-20 0.1% (v/v), stimulates Geobacillus sp. SK70

Application

Application Comment Organism
food industry potentially applied in bakery industries Geobacillus sp. SK70

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21 (DE3) Geobacillus sp. SK70

General Stability

General Stability Organism
stable in presence of 1 mM Ca2+ Geobacillus sp. SK70

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol 1% (v/v) Geobacillus sp. SK70
EDTA 1% (v/v) Geobacillus sp. SK70
SDS 1% (v/v) Geobacillus sp. SK70

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ 1 mM, stimulates Geobacillus sp. SK70
Zn2+ 1 mM, stimulates Geobacillus sp. SK70

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
135000
-
 SDS-PAGE Geobacillus sp. SK70

Organism

Organism UniProt Comment Textmining
Geobacillus sp. SK70
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Geobacillus sp. SK70

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-cyclodextrin + H2O preference of cyclodextrin as substrate over starch or pullulan Geobacillus sp. SK70 maltose + ?
-
 ?
pullulan + H2O preference of cyclodextrin as substrate over starch or pullulan Geobacillus sp. SK70 ?
-
 ?
starch + H2O preference of cyclodextrin as substrate over starch or pullulan Geobacillus sp. SK70 maltose + ?
-
 ?

Synonyms

Synonyms Comment Organism
maltogenic amylase
-
 Geobacillus sp. SK70

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
 Geobacillus sp. SK70

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
50 60 50°C: about 75% of maximal activity, 60°C: about 88% of maximal activity Geobacillus sp. SK70

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Geobacillus sp. SK70

pH Range

pH Minimum pH Maximum Comment Organism
6 8 pH 6.0: about 80% of maximal activity, pH 8.0: about 30% of maximal activity Geobacillus sp. SK70

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 10 stable Geobacillus sp. SK70