Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | about 120% activity at 1% (v/v) | Alkalihalobacillus lehensis |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Alkalihalobacillus lehensis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | about 76% residual activity at 5 mM | Alkalihalobacillus lehensis | |
Co2+ | about 13% residual activity at 5 mM | Alkalihalobacillus lehensis | |
Cu2+ | about 5% residual activity at 4 mM | Alkalihalobacillus lehensis | |
Fe2+ | about 36% residual activity at 5 mM | Alkalihalobacillus lehensis | |
Li+ | about 87% residual activity at 5 mM | Alkalihalobacillus lehensis | |
Mg2+ | about 84% residual activity at 5 mM | Alkalihalobacillus lehensis | |
additional information | not inhibited by EDTA and Tween 20 | Alkalihalobacillus lehensis | |
Ni2+ | about 2% residual activity at 5 mM | Alkalihalobacillus lehensis | |
Pb2+ | about 2% residual activity at 5 mM | Alkalihalobacillus lehensis | |
phenylmethylsulfonyl fluoride | about 2% residual activity at 1% (v/v) | Alkalihalobacillus lehensis | |
SDS | about 6% residual activity at 1% (v/v) | Alkalihalobacillus lehensis | |
Zn2+ | about 7% residual activity at 5 mM | Alkalihalobacillus lehensis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | about 162% activity at 5 mM | Alkalihalobacillus lehensis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
68000 | - |
2 * 68000, SDS-PAGE | Alkalihalobacillus lehensis |
104700 | - |
calculated from gel filtration | Alkalihalobacillus lehensis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Alkalihalobacillus lehensis | X5CPN2 | - |
- |
Alkalihalobacillus lehensis G1 | X5CPN2 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography and Superdex 200 gel filtration | Alkalihalobacillus lehensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-cyclodextrin + H2O | 80% activity compared to beta-cyclodextrin | Alkalihalobacillus lehensis | ? | - |
? | |
amylopectin + H2O | less than 2% activity compared to beta-cyclodextrin | Alkalihalobacillus lehensis | ? | - |
? | |
amylopectin + H2O | less than 2% activity compared to beta-cyclodextrin | Alkalihalobacillus lehensis G1 | ? | - |
? | |
amylose + H2O | 45% activity compared to beta-cyclodextrin | Alkalihalobacillus lehensis | ? | - |
? | |
amylose + H2O | 45% activity compared to beta-cyclodextrin | Alkalihalobacillus lehensis G1 | ? | - |
? | |
beta-cyclodextrin + H2O | 100% activity | Alkalihalobacillus lehensis | D-glucose + maltose + maltotriose + maltooligosaccharides | - |
? | |
gamma-cyclodextrin + H2O | 10% activity compared to beta-cyclodextrin | Alkalihalobacillus lehensis | ? | - |
? | |
gamma-cyclodextrin + H2O | 10% activity compared to beta-cyclodextrin | Alkalihalobacillus lehensis G1 | ? | - |
? | |
maltotriose + H2O | - |
Alkalihalobacillus lehensis | maltose + D-glucose | - |
? | |
maltotriose + H2O | - |
Alkalihalobacillus lehensis G1 | maltose + D-glucose | - |
? | |
soluble starch + H2O | 45% activity compared to beta-cyclodextrin | Alkalihalobacillus lehensis | ? | - |
? | |
soluble starch + H2O | 45% activity compared to beta-cyclodextrin | Alkalihalobacillus lehensis G1 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 68000, SDS-PAGE | Alkalihalobacillus lehensis |
Synonyms | Comment | Organism |
---|---|---|
MAG1 | - |
Alkalihalobacillus lehensis |
maltogenic amylase | - |
Alkalihalobacillus lehensis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Alkalihalobacillus lehensis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
-20 | 40 | the enzyme retains more than 50% of its activity at 40°C and 30°C for 10 min. The half-lives of the enzyme are 239.02 h, 126.03 h and 108.30 h at -20°C, 4°C and 20°C, respectively. However, the half-lives are dramatically reduced to 0.37 h and 0.04 h at 30°C and 40°C, respectively | Alkalihalobacillus lehensis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
9.9 | - |
soluble starch | at pH 7.0 and 40°C | Alkalihalobacillus lehensis | |
12.5 | - |
beta-cyclodextrin | at pH 7.0 and 40°C | Alkalihalobacillus lehensis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Alkalihalobacillus lehensis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7 | 9 | the enzyme is highly active and stable in a pH range from 7.0 to 9.0 | Alkalihalobacillus lehensis |