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Literature summary for 3.2.1.133 extracted from

  • Nasrollahi, S.; Golalizadeh, L.; Sajedi, R.H.; Taghdir, M.; Asghari, S.M.; Rassa, M.
    Substrate preference of a Geobacillus maltogenic amylase: a kinetic and thermodynamic analysis (2013), Int. J. Biol. Macromol., 60, 1-9.
    View publication on PubMed
Search for more literature for 3.2.1.133

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Geobacillus sp.

Inhibitors

Inhibitors Comment Organism Structure
Al3+ about 3.0% residual activity at 10 mM Geobacillus sp.
Ba2+ about 0.5% residual activity at 10 mM Geobacillus sp.
Ca2+ about 32% residual activity at 10 mM Geobacillus sp.
Co2+ about 1.1% residual activity at 10 mM Geobacillus sp.
Cr3+ about 22.2% residual activity at 10 mM Geobacillus sp.
EDTA complete inhibition at 10 mM Geobacillus sp.
Fe2+ about 0.4% residual activity at 10 mM Geobacillus sp.
Hg2+ about 2.7% residual activity at 10 mM Geobacillus sp.
Li+ about 41% residual activity at 10 mM Geobacillus sp.
Mg2+ about 1.4% residual activity at 10 mM Geobacillus sp.
Mn2+ about 1.5% residual activity at 10 mM Geobacillus sp.
Na+ about 1.7% residual activity at 10 mM Geobacillus sp.
Ni2+ about 0.7% residual activity at 10 mM Geobacillus sp.
Sn4+ about 89.3% residual activity at 10 mM Geobacillus sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ about 143.8% activity at 1 mM Geobacillus sp.
K+ about 186.6% activity at 1 mM Geobacillus sp.
Li+ about 166.5% activity at 1 mM Geobacillus sp.
Sn4+ about 121% activity at 1 mM Geobacillus sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
65000
-
 x * 65000, SDS-PAGE Geobacillus sp.

Organism

Organism UniProt Comment Textmining
Geobacillus sp. E0X988
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA agarose column chromatography Geobacillus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-cyclodextrin + H2O high specificity for alpha-cyclodextrin Geobacillus sp. ?
-
 ?
amylopectin + H2O
-
 Geobacillus sp. ?
-
 ?
amylose + H2O
-
 Geobacillus sp. ?
-
 ?
beta-cyclodextrin + H2O
-
 Geobacillus sp. ?
-
 ?
gamma-cyclodextrin + H2O
-
 Geobacillus sp. ?
-
 ?
glycogen + H2O
-
 Geobacillus sp. ?
-
 ?
starch + H2O
-
 Geobacillus sp. ?
-
 ?

Subunits

Subunits Comment Organism
? x * 65000, SDS-PAGE Geobacillus sp.

Synonyms

Synonyms Comment Organism
MAase
-
 Geobacillus sp.
maltogenic amylase
-
 Geobacillus sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55 65
-
 Geobacillus sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 70 about 40% activity at 40°C, about 80% activity at 50°C, 100% activity at 60°C, about 30% activity at 70°C Geobacillus sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55 75 the enzyme remains stable for up to 60 min at 55°C, after 60 min at 60 and 65°C the enzyme shows 75% activity, while after 20 min at 70 and 75°C the enzyme is inactive Geobacillus sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
15.38
-
 amylopectin in sodium-acetate buffer (50 mM, pH 6.0), at 60°C Geobacillus sp.
62.82
-
 amylose in sodium-acetate buffer (50 mM, pH 6.0), at 60°C Geobacillus sp.
225.6
-
 gamma-cyclodextrin in sodium-acetate buffer (50 mM, pH 6.0), at 60°C Geobacillus sp.
280.8
-
 beta-cyclodextrin in sodium-acetate buffer (50 mM, pH 6.0), at 60°C Geobacillus sp.
500
-
 alpha-cyclodextrin in sodium-acetate buffer (50 mM, pH 6.0), at 60°C Geobacillus sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Geobacillus sp.

pH Range

pH Minimum pH Maximum Comment Organism
5 9 about 35% activity at pH 5.0, 100% activity at pH 6.0, about 85% activity at pH 7.0, about 60% activity at pH 8.0, about 40% activity at pH 9.0 Geobacillus sp.