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Literature summary for 3.2.1.133 extracted from

  • Khemakhem, B.; Fendri, I.; Dahech, I.; Belghuith, K.; Kammoun, R.; Mejdoub, H.
    Purification and characterization of a maltogenic amylase from Fenugreek (Trigonella foenum graecum) seeds using the Box Benkhen Design (BBD) (2013), Ind. Crops Prod., 43, 334-339.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ partial inactivation Trigonella foenum-graecum
Mn2+ strong inhibition Trigonella foenum-graecum
Zn2+ strong inhibition Trigonella foenum-graecum

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ increases the activity at low concentrations (1 and 2 mM) Trigonella foenum-graecum
Fe2+ increases the activity at low concentrations (1 and 2 mM) Trigonella foenum-graecum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
68000
-
x * 68000, SDS-PAGE Trigonella foenum-graecum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
starch + H2O Trigonella foenum-graecum
-
maltose + ?
-
?

Organism

Organism UniProt Comment Textmining
Trigonella foenum-graecum
-
fenugreek
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation and MonoQ Sepharose column chromatography Trigonella foenum-graecum

Source Tissue

Source Tissue Comment Organism Textmining
seed
-
Trigonella foenum-graecum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
starch + H2O
-
Trigonella foenum-graecum maltose + ?
-
?

Subunits

Subunits Comment Organism
? x * 68000, SDS-PAGE Trigonella foenum-graecum

Synonyms

Synonyms Comment Organism
maltogenic amylase
-
Trigonella foenum-graecum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
Trigonella foenum-graecum

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 65 more than 50% activity between 40 and 65°C Trigonella foenum-graecum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
the enzyme is rapidly inactivated at temperature above 60°C and is totally inactivated at 60°C within 60 min in the absence of the substrate. However, in the presence of 1% (w/v) of soluble starch, the half time rises from approximately 10 to 60 min at 60°C Trigonella foenum-graecum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
Trigonella foenum-graecum

pH Range

pH Minimum pH Maximum Comment Organism
4 8 more than 70% activity between pH 4.0 and 7.0, about 40% activity at pH 8.0 Trigonella foenum-graecum

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 10 the enzyme retains 100% of its activity when incubated for 24 h in a pH range between 5.0 and 10.0 Trigonella foenum-graecum