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Literature summary for 3.2.1.133 extracted from

  • Cho, H.Y.; Kim, Y.W.; Kim, T.J.; Lee, H.S.; Kim, D.Y.; Kim, J.W.; Lee, Y.W.; Leed, S.; Park, K.H.
    Molecular characterization of a dimeric intracellular maltogenic amylase of Bacillus subtilis SUH4-2 (2000), Biochim. Biophys. Acta, 1478, 333-340.
    View publication on PubMed
Search for more literature for 3.2.1.133

Application

Application Comment Organism
nutrition transglycosylation activity of BbmA can be applied for the preparation of branched oligosaccharide mixtures, which are used as low calorie sweeteners or humectants Bacillus subtilis

Cloned(Commentary)

Cloned (Comment) Organism
cloned and expressed in Escherichia coli Bacillus subtilis

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Bacillus subtilis 5737
-
intracellular
-
 Bacillus subtilis 5622
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
64000
-
 SDS-PAGE Bacillus subtilis
68830
-
 predicted from nucleotide sequence data Bacillus subtilis
68900
-
 MALDI-TOF-MS spectra Bacillus subtilis
109000
-
 native BbmA, gel filtration Bacillus subtilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
starch + H2O Bacillus subtilis carbohydrate metabolism in the cytoplasm alpha-maltose + ?
-
 ?
starch + H2O Bacillus subtilis SUH4-2 carbohydrate metabolism in the cytoplasm alpha-maltose + ?
-
 ?

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
 SUH4-2
-
Bacillus subtilis SUH4-2
-
 SUH4-2
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acarbose + H2O
-
 Bacillus subtilis acarviosine-glucose + alpha-D-glucose
-
 ?
acarbose + H2O
-
 Bacillus subtilis SUH4-2 acarviosine-glucose + alpha-D-glucose
-
 ?
beta-cyclodextrin + H2O
-
 Bacillus subtilis alpha-maltose + alpha-D-glucose
-
 ?
beta-cyclodextrin + H2O
-
 Bacillus subtilis SUH4-2 alpha-maltose + alpha-D-glucose
-
 ?
pullulan + H2O
-
 Bacillus subtilis panose + ?
-
 ?
pullulan + H2O
-
 Bacillus subtilis SUH4-2 panose + ?
-
 ?
starch + H2O
-
 Bacillus subtilis alpha-maltose + ?
-
 ?
starch + H2O carbohydrate metabolism in the cytoplasm Bacillus subtilis alpha-maltose + ?
-
 ?
starch + H2O
-
 Bacillus subtilis SUH4-2 alpha-maltose + ?
-
 ?
starch + H2O carbohydrate metabolism in the cytoplasm Bacillus subtilis SUH4-2 alpha-maltose + ?
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 64000, monomer-dimer equilibrium, molar ratio 3:2, SDS-PAGE Bacillus subtilis
monomer monomer-dimer equilibrium, molar ratio 3:2 Bacillus subtilis

Synonyms

Synonyms Comment Organism
BbmA
-
 Bacillus subtilis