Literature summary for 3.2.1.132 extracted from

Chang, C.T.; Lin, Y.L.; Lu, S.W.; Huang, C.W.; Wang, Y.T.; Chung, Y.C.
Characterization of a chitosanase from Jelly Fig (Ficus awkeotsang Makino) latex and its application in the production of water-soluble low molecular weight chitosans (2016), PLoS ONE, 11, e0150490 .

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KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
additional information	-	chitosan	Km-value: 0.089 mg/ml, 50°C, pH 4.5	Ficus pumila var. awkeotsang

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
20500	-	SDS-PAGE	Ficus pumila var. awkeotsang

Organism

Organism	UniProt	Comment	Textmining
Ficus pumila var. awkeotsang	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Ficus pumila var. awkeotsang

Source Tissue

Source Tissue	Comment	Organism	Textmining
latex	-	Ficus pumila var. awkeotsang	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
aminoethyl chitosan + H2O	average molecular weight of aminoethyl low molecular weight chitosan is 8.89 kDa	Ficus pumila var. awkeotsang	aminoethyl low molecular weight chitosan + ?	-	?
carboxymethyl chitosan + H2O	average molecular weight of carboxymethyl low molecular weight chitosan is 11.2 kDa	Ficus pumila var. awkeotsang	carboxymethyl low molecular weight chitosan + ?	-	?
chitosan + H2O	the enzyme shows activity toward chitosan polymers which exhibit various degrees of deacetylation (21-94%). It hydrolyzes 70-84% deacetylated chitosan polymers most effectively. Substrate specificity analysis indicates that the enzyme catalyzes the hydrolysis of chitin and chitosan polymers and their derivatives	Ficus pumila var. awkeotsang	?	-	?
ethylene glycol chitosan + H2O	average molecular weight of ethylene glycol low molecular weight chitosan is 11.2 kDa	Ficus pumila var. awkeotsang	ethylene glycol low molecular weight chitosan + ?	-	?

Subunits

Subunits	Comment	Organism
?	x * 20500, SDS-PAGE	Ficus pumila var. awkeotsang

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
50	-	-	Ficus pumila var. awkeotsang

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
4.5	-	substrate: chitosan	Ficus pumila var. awkeotsang

pI Value

Organism	Comment	pI Value Maximum	pI Value
Ficus pumila var. awkeotsang	estimated by isoelectric focusing electrophoresis on Phast-Gel IEF 3-9	-	3.5

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Release 2023.1 (January 2023)