Any feedback?
Literature summary for 3.2.1.132 extracted from
- Improvement in the thermostability of chitosanase from Bacillus ehimensis by introducing artificial disulfide bonds (2016), Biotechnol. Lett., 38, 1809-1815 .
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| A207C/L286C | mutant enzyme has a longer half-life at 50°C (from 10.5 to 69.3 min) and a 200% higher catalytic efficiency (Kcat/Km) than that of the wild-type enzyme | Paenibacillus ehimensis |
| G113C/D116C | at 50°C the wild-type shows less than 37% of the initial activity after 30 min, mutant enzyme G113C/D116C retains 62.0% of its initial activity | Paenibacillus ehimensis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 4.8 | - |
chitosan | 40°C, pH not specified in the publication, mutant enzyme A207C/L286C, calculated as micromol reducing sugar liberated from chitosan | Paenibacillus ehimensis |  |
| 4.9 | - |
chitosan | 40°C, pH not specified in the publication, wild-type enzyme, calculated as micromol reducing sugar liberated from chitosan | Paenibacillus ehimensis | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Paenibacillus ehimensis | O24825 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| chitosan + H2O | - |
Paenibacillus ehimensis | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| chitosanase EAG1 | - |
Paenibacillus ehimensis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
30 min, wild-type shows less than 37% of the initial activity, mutant enzyme G113C/D116C retains 62.0% of its initial activity, mutant enzyme A207C//L286C retains 81.0% of its initial activity | Paenibacillus ehimensis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.5 | - |
chitosan | 40°C, pH not specified in the publication, wild-type enzyme, calculated as micromol reducing sugar liberated from chitosan | Paenibacillus ehimensis | |
| 4.9 | - |
chitosan | 40°C, pH not specified in the publication, mutant enzyme A207C/L286C, calculated as micromol reducing sugar liberated from chitosan | Paenibacillus ehimensis | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.52 | - |
chitosan | 40°C, pH not specified in the publication, wild-type enzyme, calculated as micromol reducing sugar liberated from chitosan | Paenibacillus ehimensis | |
| 1.03 | - |
chitosan | 40°C, pH not specified in the publication, mutant enzyme A207C/L286C, calculated as micromol reducing sugar liberated from chitosan | Paenibacillus ehimensis | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
