Literature summary for 3.2.1.132 extracted from

Regel, E.K.; Weikert, T.; Niehues, A.; Moerschbacher, B.M.; Singh, R.
Protein-engineering of chitosanase from Bacillus sp. MN to alter its substrate specificity (2018), Biotechnol. Bioeng., 115, 863-873 .

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Application

Application	Comment	Organism
agriculture	partially acetylated chitosan oligosaccharides have various potential applications in agriculture, biomedicine, and pharmaceutics due to their suitable bioactivities. A more promising approach is enzymatic depolymerization of chitosan using chitinases or chitosanases, as the substrate specificity of the enzyme determines the composition of the oligomeric products	Bacillus sp. MN
food industry	partially acetylated chitosan oligosaccharides have various potential applications in agriculture, biomedicine, and pharmaceutics due to their suitable bioactivities. A more promising approach is enzymatic depolymerization of chitosan using chitinases or chitosanases, as the substrate specificity of the enzyme determines the composition of the oligomeric products	Bacillus sp. MN
pharmacology	partially acetylated chitosan oligosaccharides have various potential applications in agriculture, biomedicine, and pharmaceutics due to their suitable bioactivities. A more promising approach is enzymatic depolymerization of chitosan using chitinases or chitosanases, as the substrate specificity of the enzyme determines the composition of the oligomeric products	Bacillus sp. MN

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Bacillus sp. MN

Protein Variants

Protein Variants	Comment	Organism
E309R/N319E	the mutant enzyme can accept N-acetyl-D-glucosamine units at their subsite (-2), which is impossible for the wild-type enzyme	Bacillus sp. MN
N308V/E309R/N319E	the mutant enzyme can accept N-acetyl-D-glucosamine units at their subsite (-2), which is impossible for the wild-type enzyme	Bacillus sp. MN

Organism

Organism	UniProt	Comment	Textmining
Bacillus sp. MN	H9CXV8	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
chitosan + H2O	the mutant enzymes E309R/N319E and N308V/E309R/N319E can accept N-acetyl-D-glucosamine units at their subsite (-2), which is impossible for the wild-type enzyme	Bacillus sp. MN	?	-	?

Synonyms

Synonyms	Comment	Organism
BspCsnMN	-	Bacillus sp. MN

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Release 2023.1 (January 2023)