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Literature summary for 3.2.1.132 extracted from
- Characterization of an alkalophilic extracellular chitosanase from Bacillus cereus GU-02 (2014), J. Biosci. Bioeng., 117, 684-689.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | slightly inactivated at 0.5 mM | Bacillus cereus |  |
| Mg2+ | slightly inactivated at 0.5 mM | Bacillus cereus | |
| NaCl | slightly inactivated at 0.5 mM | Bacillus cereus | |
| Zn2+ | slightly inactivated at 0.5 mM | Bacillus cereus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.000327 | - |
chitosan | at pH 8.5 and 37°C | Bacillus cereus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Bacillus cereus | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | chitosan degrading activity is enhanced by 15% in the presence of calcium ions (8 mM) | Bacillus cereus | |
| Mg2+ | slightly activated by Mn2+ | Bacillus cereus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| chitosan + H2O | Bacillus cereus | - |
chitosan oligosaccharides | chitosan oligosaccharides comprise oligomers with degree of polymerization mainly from dimers to pentamers | ? | |
| chitosan + H2O | Bacillus cereus GU-02 | - |
chitosan oligosaccharides | chitosan oligosaccharides comprise oligomers with degree of polymerization mainly from dimers to pentamers | ? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus cereus | - |
- |
- |
| Bacillus cereus GU-02 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate precipitation and Superdex 75 gel filtration | Bacillus cereus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alginate + H2O | 12.7% activity compared to chitosan | Bacillus cereus | ? | - |
? | |
| alginate + H2O | 12.7% activity compared to chitosan | Bacillus cereus GU-02 | ? | - |
? | |
| beta-glucan + H2O | 32.7% activity compared to chitosan | Bacillus cereus | ? | - |
? | |
| beta-glucan + H2O | 32.7% activity compared to chitosan | Bacillus cereus GU-02 | ? | - |
? | |
| chitosan + H2O | - |
Bacillus cereus | chitosan oligosaccharides | chitosan oligosaccharides comprise oligomers with degree of polymerization mainly from dimers to pentamers | ? | |
| chitosan + H2O | - |
Bacillus cereus GU-02 | chitosan oligosaccharides | chitosan oligosaccharides comprise oligomers with degree of polymerization mainly from dimers to pentamers | ? | |
| fucoidan + H2O | 16.9% activity compared to chitosan | Bacillus cereus | ? | - |
? | |
| fucoidan + H2O | 16.9% activity compared to chitosan | Bacillus cereus GU-02 | ? | - |
? | |
| hyaluronic acid + H2O | 26.7% activity compared to chitosan | Bacillus cereus | ? | - |
? | |
| hyaluronic acid + H2O | 26.7% activity compared to chitosan | Bacillus cereus GU-02 | ? | - |
? | |
| sulfate chitin + H2O | 9.6% activity compared to chitosan | Bacillus cereus | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| actase | - |
Bacillus cereus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Bacillus cereus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 27 | 50 | about 55% activity at 27°C, about 62% activity at 32°C, 100% activity at 37°C, about 90% activity at 42°C, and about 80% activity at 50°C | Bacillus cereus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
- |
Bacillus cereus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 11 | about 55% activity at pH 3.0, about 57% activity at pH 5.0, about 80% activity at pH 6.0, 100% activity at pH 9.0, about 75% activity at pH 10.0-11.0 | Bacillus cereus |
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