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Literature summary for 3.2.1.132 extracted from

  • Jiang, X.; Chen, D.; Chen, L.; Yang, G.; Zou, S.
    Purification, characterization, and action mode of a chitosanase from Streptomyces roseolus induced by chitin (2012), Carbohydr. Res., 355, 40-44.
    View publication on PubMed
Search for more literature for 3.2.1.132

Inhibitors

Inhibitors Comment Organism Structure
Co2+ 38% residual activity at 10 mM Streptomyces roseolus
Cu2+ 27% residual activity at 10 mM Streptomyces roseolus
EDTA 68% residual activity at 10 mM Streptomyces roseolus
Mn2+ 45% residual activity at 10 mM Streptomyces roseolus
Zn2+ 50% residual activity at 10 mM Streptomyces roseolus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ slightly activated by Mg2+ Streptomyces roseolus
additional information Ba2+ and Ca2+ show nearly no effect on relative enzymatic activity Streptomyces roseolus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
41000
-
 x * 41000, SDS-PAGE Streptomyces roseolus

Organism

Organism UniProt Comment Textmining
Streptomyces roseolus
-
-
-
Streptomyces roseolus DH
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, HiPrep Q column chromatography, and Superdex 75 gel filtration Streptomyces roseolus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chitohexaose + H2O
-
 Streptomyces roseolus chitotriose + chitobiose + chitotetraose triglucosamine is the main product ?
chitopentaose + H2O
-
 Streptomyces roseolus ?
-
 ?
chitopentaose + H2O
-
 Streptomyces roseolus DH ?
-
 ?
colloidal chitosan + H2O best substrate Streptomyces roseolus ?
-
 ?
colloidal chitosan + H2O best substrate Streptomyces roseolus DH ?
-
 ?
glycol chitin + H2O 11% activity compared to colloidal chitosan Streptomyces roseolus ?
-
 ?
glycol chitin + H2O 11% activity compared to colloidal chitosan Streptomyces roseolus DH ?
-
 ?
glycol chitosan + H2O 12% activity compared to colloidal chitosan Streptomyces roseolus ?
-
 ?
glycol chitosan + H2O 12% activity compared to colloidal chitosan Streptomyces roseolus DH ?
-
 ?
additional information the enzyme cannot hydrolyze colloidal chitin, carboxylmethyl cellulose, diglucosamine, triglucosamine, or tetraglucosamine Streptomyces roseolus ?
-
 ?
additional information the enzyme cannot hydrolyze colloidal chitin, carboxylmethyl cellulose, diglucosamine, triglucosamine, or tetraglucosamine Streptomyces roseolus DH ?
-
 ?

Subunits

Subunits Comment Organism
? x * 41000, SDS-PAGE Streptomyces roseolus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Streptomyces roseolus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
60
-
 the enzyme retains more than 80% of activity at 60°C Streptomyces roseolus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30 60 more than 90% of the initial activities are retained after incubation at 30, 40, 50, and 60°C for 30 min Streptomyces roseolus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Streptomyces roseolus

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 7 the enzyme is relatively stable at pH 5.0-7.0, retaining around 80% of initial activity for 30 min Streptomyces roseolus

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
10
-
 at pH 6.0 and 50°C Streptomyces roseolus Zn2+