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Literature summary for 3.2.1.132 extracted from

  • Oh, C.; De Zoysa, M.; Kang, D.H.; Lee, Y.; Whang, I.; Nikapitiya, C.; Heo, S.J.; Yoon, K.T.; Affan, A.; Lee, J.
    Isolation, Purification, and enzymatic characterization of extracellular chitosanase from marine bacterium Bacillus subtilis CH2 (2011), J. Microbiol. Biotechnol., 21, 1021-1025.
    View publication on PubMed
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Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
29000
-
 x * 29000, SDS-PAGE Bacillus subtilis

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-
Bacillus subtilis CH2
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation and Sephadex-G100 gel filtration Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chitosan + H2O
-
 Bacillus subtilis ?
-
 ?
chitosan + H2O
-
 Bacillus subtilis CH2 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 29000, SDS-PAGE Bacillus subtilis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Bacillus subtilis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40 60 the purified chitosanase is continuously thermostable at 40°C and the stability is gradually decreased when the temperature is increased to 50°C but rapidly decreases at 60°C Bacillus subtilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
 Bacillus subtilis

Expression

Organism Comment Expression
Bacillus subtilis enzyme expression is not induced using chitosan as a carbon source additional information
Bacillus subtilis the highest chitosanase activity is observed with fructose, and it shows high activity with other substrates such as lactose, galactose, maltose, and glucose as well up