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Literature summary for 3.2.1.132 extracted from

  • Liang, T.; Kuo, Y.; Wu, P.; Wang, C.; Dzung, N.; Wang, S.
    Purification and characterization of a chitosanase and a protease by conversion of shrimp shell wastes fermented by Serratia marcescens subsp. sakuensis TKU019 (2010), J. Chin. Chem. Soc., 57, 857-863.
No PubMed abstract available
Search for more literature for 3.2.1.132

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ 95% residual activity at 5 mM Serratia marcescens
Cu2+ 68% residual activity at 5 mM Serratia marcescens
Fe2+ 92% residual activity at 5 mM Serratia marcescens
Mn2+ 2% residual activity at 5 mM Serratia marcescens
additional information not influenced by 5 mM phenylmethylsulfonyl fluoride, 0.5 mM SDS, 0.5-2% (v/v) Tween 20, 0.5-2% (v/v) Tween 40, and 0.5-2% (v/v) Triton X-100 Serratia marcescens
SDS 57% residual activity at 2 mM Serratia marcescens
Zn2+ 72% residual activity at 5 mM Serratia marcescens

Metals/Ions

Metals/Ions Comment Organism Structure
additional information not influenced by 5 mM Ca2+ Serratia marcescens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
 gel filtration Serratia marcescens
36000
-
 1 * 36000, SDS-PAGE Serratia marcescens

Organism

Organism UniProt Comment Textmining
Serratia marcescens
-
-
-
Serratia marcescens TKU019
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, DEAE-Sepharose CL-6B column chromatography and Sephacryl S-100 gel filtration Serratia marcescens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.006
-
 culture supernatant, at pH 7.0 and 60°C Serratia marcescens
0.18
-
 after 30fold purification, at pH 7.0 and 60°C Serratia marcescens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-chitin + H2O 85% activity with chitin (beta-type) compared to chitosan (95% DD) Serratia marcescens ?
-
 ?
beta-chitin + H2O 85% activity with chitin (beta-type) compared to chitosan (95% DD) Serratia marcescens TKU019 ?
-
 ?
chitosan + H2O 100% activity with chitosan (95% degree of deacetylation), 26% activity with chitosan (85% degree of deacetylation) Serratia marcescens ?
-
 ?
chitosan + H2O 100% activity with chitosan (95% degree of deacetylation), 26% activity with chitosan (85% degree of deacetylation) Serratia marcescens TKU019 ?
-
 ?
additional information the enzyme shows no activity with chitosan (73% and 60% degrees of deacetylation), glycols chitosan, and alpha-chitin Serratia marcescens ?
-
 ?
additional information the enzyme shows no activity with chitosan (73% and 60% degrees of deacetylation), glycols chitosan, and alpha-chitin Serratia marcescens TKU019 ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 36000, SDS-PAGE Serratia marcescens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Serratia marcescens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60 80 the chitosanase maintains its initial activity at less than 60°C, the chitosanase is inactivated completely at 80°C Serratia marcescens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Serratia marcescens

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 7
-
 Serratia marcescens

Expression

Organism Comment Expression
Serratia marcescens the chitosanase is suppressed by the simultaneously existing protease which also shows the maximum activity at the third day of incubation with shrimp shell as the sole carbon/nitrogen source down