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Literature summary for 3.2.1.132 extracted from

  • Rodriguez-Martin, A.; Acosta, R.; Liddell, S.; Nunez, F.; Benito, M.J.; Asensio, M.A.
    Characterization of the novel antifungal chitosanase PgChP and the encoding gene from Penicillium chrysogenum (2010), Appl. Microbiol. Biotechnol., 88, 519-528.
    View publication on PubMed
Search for more literature for 3.2.1.132

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32000
-
 x * 32000, deduced from amino acid sequence Penicillium chrysogenum
37000
-
 x * 37000, two discrete bands appear in SDS-PAGE: an intensely stained band showing a molecular mass of about 37 kDa and a fainter band of around 40 kDa Penicillium chrysogenum
40000
-
 x * 40000, two discrete bands appear in SDS-PAGE: an intensely stained band showing a molecular mass of about 37000 Da and a fainter band of around 40000 Da Penicillium chrysogenum

Organism

Organism UniProt Comment Textmining
Penicillium chrysogenum D7R809 chitosanase preprotein
-
Penicillium chrysogenum AS51D D7R809 chitosanase preprotein
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Penicillium chrysogenum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chitosan + H2O the enzyme liberates a mean of 0.6 mM D-glucosamine per microgram of protein in 4 h reaction Penicillium chrysogenum ?
-
 ?
chitosan + H2O the enzyme liberates a mean of 0.6 mM D-glucosamine per microgram of protein in 4 h reaction Penicillium chrysogenum AS51D ?
-
 ?

Subunits

Subunits Comment Organism
? x * 30000-31000, analysis of the molecular mass by electrospray ionization-mass spectrometry reveals six major peaks from 30000 to 31000 Da that are related to different levels of glycosylation. The mass profile shows a dominant peak of 30772 Da with a series of surrounding peaks differing from each other by 162 Da Penicillium chrysogenum
? x * 32000, deduced from amino acid sequence Penicillium chrysogenum
? x * 37000, two discrete bands appear in SDS-PAGE: an intensely stained band showing a molecular mass of about 37 kDa and a fainter band of around 40 kDa Penicillium chrysogenum
? x * 40000, two discrete bands appear in SDS-PAGE: an intensely stained band showing a molecular mass of about 37000 Da and a fainter band of around 40000 Da Penicillium chrysogenum

Synonyms

Synonyms Comment Organism
PgChP
-
 Penicillium chrysogenum

pI Value

Organism Comment pI Value Maximum pI Value
Penicillium chrysogenum isoelectric focusing
-
 5