Cloned (Comment) | Organism |
---|---|
expression of GST-tagged shuffled mutant enzymes Y18 and Y20 in Escherichia coli strain BL21(DE3) | Bacillus cereus |
Protein Variants | Comment | Organism |
---|---|---|
I13T/A87V | DNA shuffling of the genes from strains KNUC51 and KNUC55, the shuffled product YM18 shows higher activity than the parents at 40°C. The specific activity of YM18 is enhanced 250% compared to the parents | Bacillus cereus |
K66R/N352S | DNA shuffling of the genes from strains KNUC51 and KNUC55, the shuffled product YM20 shows higher activity than the parents at 40°C. The specific activity of YM20 is enhanced 350% compared to the parents, YM20 exhibits a shift of the optimal pH level from pH 5.5 to pH 6.5 | Bacillus cereus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
x * 50000, recombinant detagged enzymes, SDS-PAGE, x * 81000, recombinant GST-tagged enzymes, SDS-PAGE | Bacillus cereus |
81000 | - |
x * 50000, recombinant detagged enzymes, SDS-PAGE, x * 81000, recombinant GST-tagged enzymes, SDS-PAGE | Bacillus cereus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus cereus | - |
strains KNUC51 and KNUC55, genes csn | - |
Purification (Comment) | Organism |
---|---|
recombinant GST-tagged shuffled mutant enzymes Y18 and Y20 from EScherichia coli strain BL231(DE3) by glutathione affinity chromatography. The tags are cleaved off by thrombin | Bacillus cereus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
chitosan + H2O | soluble chitosan | Bacillus cereus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 50000, recombinant detagged enzymes, SDS-PAGE, x * 81000, recombinant GST-tagged enzymes, SDS-PAGE | Bacillus cereus |
More | structure comparisons of wild-type and mutant enzymes, overview | Bacillus cereus |
Synonyms | Comment | Organism |
---|---|---|
More | the enzyme belongs to the glycohydrolase family 8 | Bacillus cereus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
wild-type enzyme | Bacillus cereus |
50 | - |
mutant YM18 | Bacillus cereus |
70 | - |
mutant YM20 | Bacillus cereus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
broader pH activity profile for YM20, in contrast to the pH activity profile of YM18, which shows the same pH optimum as the wild-type enzyme at pH 5.5 | Bacillus cereus |
5.5 | - |
wild-type enzyme | Bacillus cereus |
6.5 | - |
mutant YM20 | Bacillus cereus |