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Literature summary for 3.2.1.132 extracted from

  • Isogawa, D.; Fukuda, T.; Kuroda, K.; Kusaoke, H.; Kimoto, H.; Suye, S.; Ueda, M.
    Demonstration of catalytic proton acceptor of chitosanase from Paenibacillus fukuinensis by comprehensive analysis of mutant library (2009), Appl. Microbiol. Biotechnol., 85, 95-104.
    View publication on PubMed
Search for more literature for 3.2.1.132

Cloned(Commentary)

Cloned (Comment) Organism
subcloning and library construction in Escherichia coli strain DH5alpha, expression of the enzyme in Saccharomyces cerevisiae cells displayed at the cell surface, expression of wild-type and mutant enzymes in Saccharomyces cerevisiae Paenibacillus fukuinensis

Protein Variants

Protein Variants Comment Organism
E302A site-directed mutagenesis, the mutant enzyme shows reduced beta-1,4 glucanase activity compared to the wild-type enzyme Paenibacillus fukuinensis
E302C site-directed mutagenesis, the mutant enzyme shows reduced beta-1,4 glucanase activity compared to the wild-type enzyme Paenibacillus fukuinensis
E302D site-directed mutagenesis, the mutant enzyme shows reduced beta-1,4 glucanase activity compared to the wild-type enzyme Paenibacillus fukuinensis
E302F site-directed mutagenesis, the mutant enzyme shows reduced beta-1,4 glucanase activity compared to the wild-type enzyme Paenibacillus fukuinensis
E302G site-directed mutagenesis, the mutant enzyme shows reduced beta-1,4 glucanase activity compared to the wild-type enzyme Paenibacillus fukuinensis
E302H site-directed mutagenesis, the mutant enzyme shows reduced beta-1,4 glucanase activity compared to the wild-type enzyme Paenibacillus fukuinensis
E302I site-directed mutagenesis, the mutant enzyme shows reduced beta-1,4 glucanase activity compared to the wild-type enzyme Paenibacillus fukuinensis
E302K site-directed mutagenesis, the mutant enzyme shows reduced beta-1,4 glucanase activity compared to the wild-type enzyme Paenibacillus fukuinensis
E302K/N312A site-directed mutagenesis, the mutant enzyme shows reduced beta-1,4 glucanase activity compared to the wild-type enzyme Paenibacillus fukuinensis
E302K/N312D site-directed mutagenesis, the mutant enzyme shows reduced beta-1,4 glucanase activity compared to the wild-type enzyme Paenibacillus fukuinensis
E302K/N312K site-directed mutagenesis, the mutant enzyme shows no beta-1,4 glucanase activity Paenibacillus fukuinensis
E302K/N312R site-directed mutagenesis, the mutant enzyme shows no beta-1,4 glucanase activity Paenibacillus fukuinensis
E302L site-directed mutagenesis, the mutant enzyme shows reduced beta-1,4 glucanase activity compared to the wild-type enzyme Paenibacillus fukuinensis
E302M site-directed mutagenesis, the mutant enzyme shows reduced beta-1,4 glucanase activity compared to the wild-type enzyme Paenibacillus fukuinensis
E302N site-directed mutagenesis, the mutant enzyme shows reduced beta-1,4 glucanase activity compared to the wild-type enzyme Paenibacillus fukuinensis
E302P site-directed mutagenesis, the mutant enzyme shows reduced beta-1,4 glucanase activity compared to the wild-type enzyme Paenibacillus fukuinensis
E302Q site-directed mutagenesis, the mutant enzyme shows reduced beta-1,4 glucanase activity compared to the wild-type enzyme Paenibacillus fukuinensis
E302R site-directed mutagenesis, the mutant enzyme shows reduced beta-1,4 glucanase activity compared to the wild-type enzyme Paenibacillus fukuinensis
E302R/N312A site-directed mutagenesis, the mutant enzyme shows no beta-1,4 glucanase activity Paenibacillus fukuinensis
E302R/N312D site-directed mutagenesis, the mutant enzyme shows reduced beta-1,4 glucanase activity compared to the wild-type enzyme Paenibacillus fukuinensis
E302R/N312K site-directed mutagenesis, the mutant enzyme shows no beta-1,4 glucanase activity Paenibacillus fukuinensis
E302R/N312R site-directed mutagenesis, the mutant enzyme shows no beta-1,4 glucanase activity Paenibacillus fukuinensis
E302S site-directed mutagenesis, the mutant enzyme shows reduced beta-1,4 glucanase activity compared to the wild-type enzyme Paenibacillus fukuinensis
E302T site-directed mutagenesis, the mutant enzyme shows reduced beta-1,4 glucanase activity compared to the wild-type enzyme Paenibacillus fukuinensis
E302V site-directed mutagenesis, the mutant enzyme shows reduced beta-1,4 glucanase activity compared to the wild-type enzyme Paenibacillus fukuinensis
E302Y site-directed mutagenesis, the mutant enzyme shows reduced beta-1,4 glucanase activity compared to the wild-type enzyme Paenibacillus fukuinensis
additional information establishing of a recombinant expression of the enzyme at the cell surface of Saccharomyces cerevisiae strain MT8-1 cells, using a yeast cell surface-displaying system, to facilitate enzyme purification Paenibacillus fukuinensis
N312A site-directed mutagenesis, the mutant enzyme shows reduced beta-1,4 glucanase activity compared to the wild-type enzyme Paenibacillus fukuinensis
N312D site-directed mutagenesis, the mutant enzyme shows reduced beta-1,4 glucanase activity compared to the wild-type enzyme Paenibacillus fukuinensis
N312K site-directed mutagenesis, the mutant enzyme shows reduced beta-1,4 glucanase activity compared to the wild-type enzyme Paenibacillus fukuinensis
N312R site-directed mutagenesis, the mutant enzyme shows reduced beta-1,4 glucanase activity compared to the wild-type enzyme Paenibacillus fukuinensis

Organism

Organism UniProt Comment Textmining
Paenibacillus fukuinensis
-
-
-
Paenibacillus fukuinensis D2
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glycol chitosan + H2O
-
 Paenibacillus fukuinensis ?
-
 ?
glycol chitosan + H2O
-
 Paenibacillus fukuinensis D2 ?
-
 ?
additional information the chitosanase from Paenibacillus fukuinensis exhibits both chitosanase and beta-1,4 glucanase activities. Glu302 is a proton acceptor for chitosanase activity, and Asn312 also participates in the hydrolysis of chitosan and cellulose Paenibacillus fukuinensis ?
-
 ?
additional information the chitosanase from Paenibacillus fukuinensis exhibits both chitosanase and beta-1,4 glucanase activities. Glu302 is a proton acceptor for chitosanase activity, and Asn312 also participates in the hydrolysis of chitosan and cellulose Paenibacillus fukuinensis D2 ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Paenibacillus fukuinensis