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Literature summary for 3.2.1.132 extracted from

  • Sun, Y.; Zhang, J.; Wang, S.
    Purification and characterization of the chitosanase from Aeromonas sp. HG08 (2009), Afr. J. Biotechnol., 8, 2830-2834.
No PubMed abstract available
Search for more literature for 3.2.1.132

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol activates 28% at 1 mM Aeromonas sp.

Inhibitors

Inhibitors Comment Organism Structure
Ag+ inhibits 60% at 1 mM Aeromonas sp.
Cu2+ inhibits 25% at 1 mM Aeromonas sp.
EDTA inhibits 18% at 1 mM Aeromonas sp.
Fe3+ inhibits 60% at 1 mM Aeromonas sp.
Hg2+ inhibits 37% at 1 mM Aeromonas sp.
additional information PMSF has no effect on enzyme activity Aeromonas sp.
SDS inhibits 21% at 1 mM Aeromonas sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular the enzyme is secreted Aeromonas sp.
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ activates 46% at 1 mM Aeromonas sp.
additional information PMSF, K+, Na+, Pb2+, Mg2+, Ba2+ and Zn2+ have no effect on enzyme activity Aeromonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
70000
-
 gel filtration Aeromonas sp.

Organism

Organism UniProt Comment Textmining
Aeromonas sp.
-
-
-
Aeromonas sp. HG08
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 21.25fold Aeromonas sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
850
-
 purified enzyme Aeromonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chitosan + H2O 70-100% deacetylated chitosan, the activity of AsChi increases with the degree of deacetylation of chitosan. AsChi probably hydrolyzes chitosan in an endo-type fashion Aeromonas sp. ?
-
 ?
chitosan + H2O 70-100% deacetylated chitosan, the activity of AsChi increases with the degree of deacetylation of chitosan. AsChi probably hydrolyzes chitosan in an endo-type fashion Aeromonas sp. HG08 ?
-
 ?
additional information no activity with 50% deacetylated chitosan, 19% deacetylated colloidal chitin, carboxymethylcellulose sodium, and carboxymethyl chitosan Aeromonas sp. ?
-
 ?
additional information no activity with 50% deacetylated chitosan, 19% deacetylated colloidal chitin, carboxymethylcellulose sodium, and carboxymethyl chitosan Aeromonas sp. HG08 ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 70000, SDS-PAGE Aeromonas sp.

Synonyms

Synonyms Comment Organism
AsChi
-
 Aeromonas sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
 Aeromonas sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20
-
 purified enzyme, pH 5.8, 2 h, 98% activity remaining Aeromonas sp.
30
-
 purified enzyme, pH 5.8, 2 h, 92% activity remaining Aeromonas sp.
40
-
 purified enzyme, pH 5.8, 2 h, 85% activity remaining Aeromonas sp.
50
-
 purified enzyme, pH 5.8, half-live is 30 min Aeromonas sp.
60
-
 purified enzyme, pH 5.8, half-live is 13 min Aeromonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.2
-
-
 Aeromonas sp.