Literature summary for 3.2.1.132 extracted from

Saito, A.; Ooya, T.; Miyatsuchi, D.; Fuchigami, H.; Terakado, K.; Nakayama, S.Y.; Watanabe, T.; Nagata, Y.; Ando, A.
Molecular characterization and antifungal activity of a family 46 chitosanase from Amycolatopsis sp. CsO-2 (2009), FEMS Microbiol. Lett., 293, 79-84.

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Cloned(Commentary)

Cloned (Comment)	Organism
wild-type and mutant overexpressed from vector pET15b/CSO2 in Escherichia coli BL21(DE3)	Amycolatopsis sp. CsO-2

Protein Variants

Protein Variants	Comment	Organism
E22Q	abolished chitosanase activity, does not exhibit antifungal activity	Amycolatopsis sp. CsO-2

Inhibitors

Inhibitors	Comment	Organism	Structure
additional information	antifungal effect of CtoA against Rhizopus oryzae IAM6252 is drastically enhanced by the simultaneous addition of the family 19 chitinase ChiC from Streptomyces griseus	Amycolatopsis sp. CsO-2

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
26490	-	sequence analysis	Amycolatopsis sp. CsO-2
30000	-	1 * 30000, SDS-PAGE	Amycolatopsis sp. CsO-2

Organism

Organism	UniProt	Comment	Textmining
Amycolatopsis sp. CsO-2	Q9LBG4	-	-

Purification (Commentary)

Purification (Comment)	Organism
to homogeneity	Amycolatopsis sp. CsO-2

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
chitosan + H2O	exhibits antifungal activity against Rhizopus oryzae IAM6252, which is known to produce chitosan probably as a cell wall component. Glu22 acts as an active center in the CtoA protein	Amycolatopsis sp. CsO-2	?	-	?

Subunits

Subunits	Comment	Organism
monomer	1 * 30000, SDS-PAGE	Amycolatopsis sp. CsO-2

Synonyms

Synonyms	Comment	Organism
CtoA	-	Amycolatopsis sp. CsO-2
family 46 chitosanase	-	Amycolatopsis sp. CsO-2

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Release 2023.1 (January 2023)