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Literature summary for 3.2.1.132 extracted from

  • Ando, A.; Saito, A.; Arai, S.; Usuda, S.; Furuno, M.; Kaneko, N.; Shida, O.; Nagata, Y.
    Molecular characterization of a novel family-46 chitosanase from Pseudomonas sp. A-01 (2008), Biosci. Biotechnol. Biochem., 72, 2074-2081.
    View publication on PubMed
Search for more literature for 3.2.1.132

Cloned(Commentary)

Cloned (Comment) Organism
wild-type and mutants overexpressed in Brevibacillus choshinensis HDP31-M3 carrying the cto1 gene on expression plasmid vector pNCMO2 Pseudomonas sp. A-01

Protein Variants

Protein Variants Comment Organism
D41N hydrolyzing activity against 100% deacetylated chitin is severely affected Pseudomonas sp. A-01
E23Q hydrolyzing activity against 100% deacetylated chitin is severely affected Pseudomonas sp. A-01

Inhibitors

Inhibitors Comment Organism Structure
Cd2+ 1 mM inhibits by 36%, with colloidal chitosan as substrate Pseudomonas sp. A-01
Co2+ 1 mM inhibits by 86%, with colloidal chitosan as substrate Pseudomonas sp. A-01
Cs+ 1 mM inhibits by 22%, with colloidal chitosan as substrate Pseudomonas sp. A-01
Cu2+ 1 mM completely inhibits, with colloidal chitosan as substrate Pseudomonas sp. A-01
Hg2+ 1 mM completely inhibits, with colloidal chitosan as substrate Pseudomonas sp. A-01
Mn2+ 1 mM inhibits by 30%, with colloidal chitosan as substrate Pseudomonas sp. A-01
additional information 1 mM Mg2+, Ca2+, Fe3+, Zn2+, Ba2+ and Pb2+ have little or no effect on activity Pseudomonas sp. A-01
Ni2+ 1 mM inhibits by 84%, with colloidal chitosan as substrate Pseudomonas sp. A-01

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25590
-
 sequence analysis Pseudomonas sp. A-01
28000
-
 purified protein Pseudomonas sp. A-01

Organism

Organism UniProt Comment Textmining
Pseudomonas sp. A-01 Q8KZM5
-
-

Purification (Commentary)

Purification (Comment) Organism
by two consecutive anion exchange chromatography steps and subsequent reverse-phase column chromatography, to homogeneity, 65fold with a yield of 1.7% Pseudomonas sp. A-01

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.06
-
 culture supernatant Pseudomonas sp. A-01
3.9
-
 65fold purified enzyme Pseudomonas sp. A-01

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
colloidal chitosan + H2O 100% deacetylated chitin Pseudomonas sp. A-01 ?
-
 ?
glycol chitosan + H2O activity is ca. 4fold higher than toward chitosan Pseudomonas sp. A-01 ?
-
 ?
N-acetylated chitosan + H2O activity is ca. 3fold less than toward chitosan Pseudomonas sp. A-01 ?
-
 ?

Synonyms

Synonyms Comment Organism
chitosanase 1
-
 Pseudomonas sp. A-01
Cto1
-
 Pseudomonas sp. A-01

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Pseudomonas sp. A-01

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 8 purified enzyme Pseudomonas sp. A-01