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Literature summary for 3.2.1.132 extracted from
- Directed evolution to enhance secretion efficiency and thermostability of chitosanase from Mitsuaria chitosanitabida 3001 (2006), Biosci. Biotechnol. Biochem., 70, 559-563.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| G151D | inactive. Use of strain for isolation of mutant genes with restored activity | Mitsuaria chitosanitabida |
| G151D/N222S | mutant with restored activity based on inactive mutant G151D, 1.2 fold higher in specific activity than wild-type and 17% increase in thermal stability at 50°C | Mitsuaria chitosanitabida |
| L74Q/V75I/G151D | mutant with restored activity based on inactive mutant G151D, 1.5fold higher in specfic activity than wild-type, and protein is efficiently secreted | Mitsuaria chitosanitabida |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mitsuaria chitosanitabida | - |
- |
- |
| Mitsuaria chitosanitabida 3001 | - |
- |
- |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Mitsuaria chitosanitabida |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
1 h, 25% residual activity for wild-type, 27% residual activity for mutant L74Q/V75I/G151D, 42% residual activity for mutant G151D/N222S | Mitsuaria chitosanitabida |
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Release 2023.1 (January 2023)
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