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Literature summary for 3.2.1.132 extracted from

  • Kim, P.I.; Kang, T.H.; Chung, K.J.; Kim, I.S.; Chung, K.C.
    Purification of a constitutive chitosanase produced by Bacillus sp. MET 1299 with cloning and expression of the gene (2004), FEMS Microbiol. Lett., 240, 31-39.
    View publication on PubMed
Search for more literature for 3.2.1.132

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Bacillus sp. (in: Bacteria)

Inhibitors

Inhibitors Comment Organism Structure
Fe2+ 1 mM, 60% inhibition Bacillus sp. (in: Bacteria)
Hg2+ 1 mM, 44% inhibition Bacillus sp. (in: Bacteria)

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Bacillus sp. (in: Bacteria)
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 1 mM, 20% activation Bacillus sp. (in: Bacteria)
Mn2+ 1 mM, 37% activation Bacillus sp. (in: Bacteria)

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
52000
-
 x * 52000, SDS-PAGE Bacillus sp. (in: Bacteria)

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Bacillus sp. (in: Bacteria) the enzyme is constitutively produced ?
-
 ?
additional information Bacillus sp. (in: Bacteria) MET 1299 the enzyme is constitutively produced ?
-
 ?

Organism

Organism UniProt Comment Textmining
Bacillus sp. (in: Bacteria)
-
-
-
Bacillus sp. (in: Bacteria) MET 1299
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Bacillus sp. (in: Bacteria)

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
476
-
-
 Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chitosan + H2O high activity on 90% deacetylated colloidal chitosan Bacillus sp. (in: Bacteria) chitohexaose + chitopentaose + chitotetraose + chitotriose + chitobiose chitosan is hydrolyzed to chitohexaose and chitopentaose at the initial stage of the reaction, after incubation for 1 h, the amounts of chitobiose, chitotriose and chitotetraose in the hydrolysate is increased ?
chitosan + H2O high activity on 90% deacetylated colloidal chitosan Bacillus sp. (in: Bacteria) MET 1299 chitohexaose + chitopentaose + chitotetraose + chitotriose + chitobiose chitosan is hydrolyzed to chitohexaose and chitopentaose at the initial stage of the reaction, after incubation for 1 h, the amounts of chitobiose, chitotriose and chitotetraose in the hydrolysate is increased ?
additional information the enzyme is constitutively produced Bacillus sp. (in: Bacteria) ?
-
 ?
additional information no activity on colloidal chitin Bacillus sp. (in: Bacteria) ?
-
 ?
additional information the enzyme is constitutively produced Bacillus sp. (in: Bacteria) MET 1299 ?
-
 ?
additional information no activity on colloidal chitin Bacillus sp. (in: Bacteria) MET 1299 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 52000, SDS-PAGE Bacillus sp. (in: Bacteria)

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Bacillus sp. (in: Bacteria)

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 70 40°C: about 40% of maximal activity, 70°C: about 15% of maximal activity Bacillus sp. (in: Bacteria)

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 pH 5.5-9.0, stable below Bacillus sp. (in: Bacteria)

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
 Bacillus sp. (in: Bacteria)

pH Range

pH Minimum pH Maximum Comment Organism
4 6 pH 4.0: about 45% of maximal activity, pH 6.0: about 25% of maximal activity Bacillus sp. (in: Bacteria)

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.5 9 stable at temperatures below 60°C Bacillus sp. (in: Bacteria)