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Literature summary for 3.2.1.132 extracted from
- Purification and characterization of hydrolase with chitinase and chitosanase activity from commercial stem bromelain (2002), J. Agric. Food Chem., 50, 4666-4673.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | - |
Ananas comosus |  |
| Hg2+ | - |
Ananas comosus | |
| N-bromosuccinimide | - |
Ananas comosus | |
| p-hydroxymercuribenzoic acid | - |
Ananas comosus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | 0.088 mg/ml chitosan | Ananas comosus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 15800 | - |
x * 15800, SDS-PAGE | Ananas comosus |
| 31400 | - |
gel filtration | Ananas comosus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| chitosan + H2O | Ananas comosus | - |
? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Ananas comosus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Ananas comosus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 444 | - |
after Superdex 75 HR gel filtration | Ananas comosus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| chitosan + H2O | - |
Ananas comosus | ? | - |
? | |
| chitosan + H2O | - |
Ananas comosus | additional information | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 15800, SDS-PAGE | Ananas comosus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Ananas comosus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 3 | - |
- |
Ananas comosus |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Ananas comosus | isoelectric focusing | - |
5.9 |
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Release 2023.1 (January 2023)
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