KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | KM is 5.2 mg/ml for PSC-I, 4 mg/ml for PSC-II and 5.6 mg/ml for PSC-III | Sus scrofa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
31000 | - |
enzyme from males and winged females | Macrotermes estherae |
35000 | - |
enzyme from soldiers | Macrotermes estherae |
37500 | - |
gel filtration, isoform PSC-I | Sus scrofa |
40000 | - |
enzyme from workers | Macrotermes estherae |
40000 | - |
1 * 40000, SDS-PAGE | Sus scrofa |
40600 | - |
gel filtration, isoform PSC-II | Sus scrofa |
43700 | - |
gel filtration, isoform PSC-III | Sus scrofa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
chitosan + H2O | Sus scrofa | - |
? | - |
? | |
chitosan + H2O | Macrotermes estherae | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Macrotermes estherae | - |
workers, soldiers, males, winged females | - |
Sus scrofa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Sus scrofa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | in crude pepsin preparation | Sus scrofa | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
489.8 | - |
isoenzyme PSC-II | Sus scrofa |
733.5 | - |
isoenzyme PSC-III | Sus scrofa |
742.6 | - |
isoenzyme PSC-I | Sus scrofa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
chitosan + H2O | - |
Sus scrofa | ? | - |
? | |
chitosan + H2O | - |
Macrotermes estherae | ? | - |
? | |
chitosan + H2O | - |
Macrotermes estherae | additional information | - |
? | |
chitosan + H2O | most effective with 68-88% deacetylated chitosan as substrate | Sus scrofa | additional information | - |
? | |
N,N'',N'''-triacetylchitotriose + H2O | - |
Sus scrofa | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 40000, SDS-PAGE | Sus scrofa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
23 | 25 | enzyme from soldiers | Macrotermes estherae |
24 | - |
enzyme from males | Macrotermes estherae |
24 | 26 | enzyme from workers | Macrotermes estherae |
25 | - |
enzyme from winged females | Macrotermes estherae |
30 | - |
PSC-III | Sus scrofa |
40 | - |
PSC-I and PSC-II | Sus scrofa |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 70 | all three isoforms are stable in this range for 30 min | Sus scrofa |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.3 | - |
chitosan | 40°C, pH 4, isoform PSC-II | Sus scrofa | |
5.9 | - |
chitosan | 40°C, pH 4, isoform PSC-I | Sus scrofa | |
6.2 | - |
chitosan | 40°C, pH 4, isoform PSC-III | Sus scrofa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | - |
isoenzyme PSC-III | Sus scrofa |
4.9 | - |
enzyme from males | Macrotermes estherae |
5 | - |
isoenzymes PSC-I and PSC-II | Sus scrofa |
5.3 | - |
enzyme from winged females | Macrotermes estherae |
5.8 | - |
enzyme from soldiers | Macrotermes estherae |
6.2 | - |
enzyme from workers | Macrotermes estherae |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | isoenzyme PSC-III is stable in this range | Sus scrofa |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3 | 7 | isoenzyme PSC-II is stable in this range | Sus scrofa |
5 | 8 | isoenzyme PSC-I is stable in this range | Sus scrofa |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Sus scrofa | isoelectrofocusing, PSC-III | - |
4.4 |
Sus scrofa | isoelectrofocusing, PSC-II | 4.7 | 4.6 |
Sus scrofa | isoelectrofocusing, PSC-I | - |
4.9 |