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Literature summary for 3.2.1.132 extracted from
- Purification and properties of two endochitosanases from Mucor rouxii implicated in its cell wall degradation (1992), FEMS Microbiol. Lett., 95, 187-194.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| acetate | enzyme A, enzyme B is not affected | Amylomyces rouxii |  |
| chitosan | substrate inhibition, enzyme B | Amylomyces rouxii | |
| Cu2+ | - |
Amylomyces rouxii | |
| Hg2+ | - |
Amylomyces rouxii | |
| Mn2+ | - |
Amylomyces rouxii | |
| Pb2+ | - |
Amylomyces rouxii | |
| Sn2+ | - |
Amylomyces rouxii | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Amylomyces rouxii |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | activates enzyme B | Amylomyces rouxii | |
| K+ | activates enzyme A | Amylomyces rouxii | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 63000 | - |
enzyme B, gel filtration | Amylomyces rouxii |
| 79000 | - |
enzyme A, gel filtration | Amylomyces rouxii |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| chitosan + H2O | Amylomyces rouxii | - |
? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Amylomyces rouxii | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 2 forms: A and B | Amylomyces rouxii |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 42.7 | - |
after second Mono Q HR 5/5 chromatography, enzyme A | Amylomyces rouxii |
| 102.3 | - |
after Superose 6 HR 10/30 chromatography, enzyme B | Amylomyces rouxii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| chitosan + H2O | - |
Amylomyces rouxii | ? | - |
? | |
| chitosan + H2O | hydrolysis of: chitosan extracted from Mucor rouxii cell walls | Amylomyces rouxii | additional information | mixture of dimer, trimer and higher molecular weight oligomers of glucosamine | ? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
enzyme B | Amylomyces rouxii |
| 55 | - |
enzyme A | Amylomyces rouxii |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
pH 5.0, 30 min, stable | Amylomyces rouxii |
| 40 | - |
pH 5.0, 30 min, stable | Amylomyces rouxii |
| 60 | - |
30 min, pH 5.0, complete inactivation | Amylomyces rouxii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Amylomyces rouxii |
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